Field evaluation of resistance of transgenic rice containing a synthetic cry1Ab gene from Bacillus thuringiensis Berliner to two stem borers
Field evaluation of resistance of transgenic rice containing a synthetic cry1Ab gene from Bacillus thuringiensis Berliner to two stem borers
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DOI：
10.1603/0022-0493-94.1.271
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发表时间：
2001-02-01
影响因子：
2.2
通讯作者：
Altosaar, I
中科院分区：
文献类型：
--
作者：
Ye, GY;Shu, QY;Altosaar, I
两个转基因水稻（Oryza sativa L.）品系，即 R-4 代的 KMD1 和 KMD2，用来自苏云金芽孢杆菌的合成 cly1Ab 基因转化，首次在 1998 年水稻生长季期间在中国浙江省两个地区的田间评估了螟虫抗性。 KMD1和KMD2均对二化螟和二化螟具有高度抗性，并且在整个水稻生长季节完全不受损害。相反，未转化的亲本对照（秀水11）对植物的损害是枯心病或白头病。在二化螟自然侵染下，对对照植物的损害达到峰值，88.7%的植物和20.1%的分蘖遇到死心。新生条纹螟在营养期和孕穗期的人工和自然侵染下，人工侵染对照植物中分别有100%的植株和25.6%的分蘖出现白头病症状，78.9%的植株和15.6%的分蘖出现白头病症状。三叶草人工侵染对对照植物的损害达到峰值，97.0% 的植物和 22.9% 的分蘖受损。田间研究表明，KMD1 和 KMD2 都显示出保护水稻免受这两种螟虫攻击的巨大潜力。
Two transgenic rice (Oryza sativa L.) lines, KMD1 and KMD2 at the R-4 generation, transformed with a synthetic cly1Ab gene from Bacillus thuringiensis Berliner, were first evaluated for stem borer resistance in the field during the rice growing season of 1998 in two areas of Zhejiang Province, China. Both KMD1 and KMD2 were highly resistant to the stem borers Chilo suppressalis (Walker) and Scirpophaga incertulas (Walker), and were completely undamaged during the whole rice growing season. In contrast, damage to the plants of the untransformed parental control (Xiushui 11) was in the form of deadhearts or whiteheads. Under natural infestation by the C. suppressalis, the damage to control plants reached a peak of 88.7% of plants and 20.1% of tillers encountered with deadhearts. Under artificial and natural infestation of neonate striped stem borers at the vegetative stage and booting stage, 100% of plants and 25.6% of tillers, 78.9% of plants and 15.6% of productive tillers among artificially infested control plants were observed with the symptom of deadhearts and whiteheads, respectively. Damage to the control plants from artificial infestation by the S. incertulas reached a peak of 97.0% of plants and 22.9% of tillers damaged. The field research indicated that both KMD1 and KMD2 show great potential for protecting rice from attack by these two stem borers.