New insights into the dual recruitment of IgA+ B cells in the developing mammary gland
New insights into the dual recruitment of IgA+ B cells in the developing mammary gland
复制标题
DOI：
10.1016/j.molimm.2008.04.017
复制
发表时间：
2008-07-01
影响因子：
3.6
通讯作者：
Salmon, Henri
中科院分区：
文献类型：
--
作者：
Bourges, Dorothee;Meurens, Francois;Salmon, Henri
在单胃哺乳动物中，通过牛奶和初乳的被动免疫转移在保护新生儿免受粘膜感染中起着重要作用。在这里，我们分析了以下假设：在妊娠/哺乳期IgA（+）浆膜期间，静脉曲张将肠道和呼吸表面留在乳腺（MG）。我们比较了表达归因受体alpha 4 beta 1和alpha 4 beta 7的淋巴细胞的募集，以表达其血管反受体VCAM-1和MADCAM-1。此外，分析了负责募集IgA（+）浆液的趋化因子的表达。数据证实，在怀孕期间，CCL28和MADCAM-1的表达升高，α4β1（+）和α4β7β7（+）/IgA（+）/IgA（+）细胞募集的泌乳中与CCL28表达的增加相关。有趣的是，在哺乳猪毫克的小血管中发现了VCAM-1的表达，而在MIC的小鼠中，VCAM-1在Mg内的大血管中表达。因此，我们的结果表明，IgA（+）浆液募集到Mg的募集是由VCAM-1/Alpha 4 beta 1和MadCAM-1/Alpha 4 beta 7与CCL28/CCR10介导的。它们支持在肠道和上呼吸道表面和毫克之间存在功能联系，从而对新生儿中的空气消化病原体进行保护。 （c）2008 Elsevier Ltd.保留所有权利。
In monogastric mammals, transfer of passive immunity via milk and colostrum plays an important role in protecting the neonate against mucosal infections. Here we analyzed the hypothesis that during gestation/lactation IgA(+) plasmablasts leave the intestinal and respiratory surfaces towards the mammary gland (MG). We compared the recruitment of lymphocytes expressing homing receptors alpha 4 beta 1 and alpha 4 beta 7 to expression of their vascular counter-receptors, VCAM-1 and MAdCAM-1. Furthermore, the expression of the chemokines responsible for the recruitment of IgA(+) plasmablasts was analyzed. Data confirmed that expressions of CCL28 and MAdCAM-1 in the MG increased during pregnancy and alpha 4 beta 1(+) and alpha 4 beta 7(+)/IgA(+) cell recruitment in lactation correlated with increase of CCL28 expression. Interestingly, VCAM-1 expression was found in small blood vessels of the lactating porcine MG, while in mice VCAM-1 was expressed in large blood vessels within the MG. Thus, our results indicate that the recruitment of IgA(+) plasmablasts to MG is mediated by VCAM-1/alpha 4 beta 1 and MAdCAM-1/alpha 4 beta 7 in conjunction with CCL28/CCR10. They support the existence of a functional link between entero- and upper respiratory surfaces and MG, thereby, conferring protection against aero-digestive pathogens in the newborn. (C) 2008 Elsevier Ltd. All rights reserved.