Using polymeric materials to generate an amplified response to molecular recognition events
Using polymeric materials to generate an amplified response to molecular recognition events
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DOI：
10.1038/nmat2042
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发表时间：
2008-01-01
期刊：
影响因子：
41.2
通讯作者：
Bowman, Christopher N.
中科院分区：
文献类型：
--
作者：
Sikes, Hadley D.;Hansen, Ryan R.;Bowman, Christopher N.
临床和现场便携式诊断设备需要快速、轻松且低成本地检测阿托摩尔至泽摩尔的生物分子，并且在稳健性和可靠性方面具有严格的要求。尽管已经报道了解决这一难题的许多创造性方法(1-9)，但市场上仍然存在许多未满足的需求，特别是在资源匮乏的环境中(10-12)。通过合理的材料设计，我们研究了利用自由基链聚合反应固有的放大来检测分子识别。基于聚合的扩增显示出可产生宏观可观察的聚合物，肉眼容易看到，这是由于类似 1,000 个识别事件（10 zeptomoles）的结果。设计和合成能够选择性识别和引发聚合反应的双功能大分子对于获得高灵敏度和消除对任何检测设备的需求至关重要。在此，我们详细介绍了所使用的设计标准，并将我们的发现与使用酶扩增获得的结果进行比较。最令人兴奋的是，这种新方法具有通用性，因为它很容易适应任何类型的特定生物相互作用的水平非常低的情况下的轻松检测。
Clinical and field-portable diagnostic devices require the detection of atto- to zeptomoles of biological molecules rapidly, easily and at low cost, with stringent requirements in terms of robustness and reliability. Though a number of creative approaches to this diffiult problem have been reported(1-9), numerous unmet needs remain in the marketplace, particularly in resource-poor settings(10-12). Using rational materials design, we investigated harnessing the amplification inherent in a radical chain polymerization reaction to detect molecular recognition. Polymerization-based amplification is shown to yield a macroscopically observable polymer, easily visible to the unaided eye, as a result of as few as similar to 1,000 recognition events (10 zeptomoles). Design and synthesis of a dual-functional macromolecule that is capable both of selective recognition and of initiating a polymerization reaction was central to obtaining high sensitivity and eliminating the need for any detection equipment. Herein, we detail the design criteria that were used and compare our findings with those obtained using enzymatic amplification. Most excitingly, this new approach is general in that it is readily adaptable to facile detection at very low levels of specific biological interactions of any kind.