Molecular response of mitochondria to a short-duration femtosecond-laser stimulation.
Molecular response of mitochondria to a short-duration femtosecond-laser stimulation.
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DOI：
10.1364/boe.8.004965
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发表时间：
2017-11
影响因子：
3.4
通讯作者：
Yujie Zhu;Hao He
中科院分区：
文献类型：
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作者：
Yujie Zhu;Hao He
线粒体功能障碍的研究具有重要意义，并与一系列神经退行性疾病有关。传统上，为了研究线粒体的动力学和功能，线粒体通常通过用外源化学物质(如氧化剂)处理细胞来间接刺激。这种治疗缺乏精确度和可控性，同时会激活未知的复杂细胞过程。在这项研究中，我们报告了飞秒激光双光子100-μS线扫描可以诱导任何目标线粒体的可修复的碎裂或肿胀，而不是消融或破坏。它可以通过定制的双光子线扫描来定义，并作为单个帧插入到任何显微镜序列中。线粒体的反应依赖于激光脉冲的峰值功率、细胞的氧化环境和线粒体膜通透性转换孔。光刺激可以调节细胞色素C和Bax的转运。此外，如果整个细胞受到刺激，则可以观察到显著上调Bcl2的表达。这些结果表明线粒体和分子对光刺激的反应是相当复杂的。这种飞秒激光刺激方法可以为相关的生物学研究提供一种非常无创、精确和可控的刺激单靶线粒体的方法。
The research of mitochondrial dysfunction is of great importance and implicated in a range of neurodegenerative diseases. Traditionally, to investigate mitochondrial dynamics and functions, mitochondria are usually stimulated indirectly by treating cells with exogenous chemicals like oxidative agents. Such treatment lacks precision and controllability, and will simultaneously activate unknown complex cell processes. In this study, we report that two-photon 100-μs line scan by a femtosecond laser can induce restorable fragmentation or swelling of any targeted mitochondria instead of ablation or disruption. It can be defined by a customized two-photon line scan and inserted into any microscopy sequence as a single frame. The mitochondrial response is dependent on the peak power of laser pulses, cellular oxidative environment, and membrane permeability transition pores of mitochondria. The translocation of cytochrome C and Bax can be regulated by the photostimulation. Moreover, significant upregulation of Bcl-2 can be observed if the whole cell is stimulated. Those results suggest the mitochondrial and molecular response to photostimulation is quite complex. This femtosecond-laser stimulation method can thus provide a very noninvasive, precise, and controllable method to stimulate single target mitochondria for related biological research.