Hypotensive and vasorelaxant effects of the procyanidin fraction from Guazuma ulmifolia bark in normotensive and hypertensive rats
Hypotensive and vasorelaxant effects of the procyanidin fraction from Guazuma ulmifolia bark in normotensive and hypertensive rats
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DOI：
10.1016/j.jep.2008.01.015
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发表时间：
2008-04-17
影响因子：
5.4
通讯作者：
Guillermo Enriquez, Raul
中科院分区：
文献类型：
--
作者：
Alfonso Magos, Gil;Carlos Mateos, Juan;Guillermo Enriquez, Raul
研究的目的：研究了从传统上用作抗高血压药物的Guazuma ulmifolia树皮丙酮提取物中获得的原花青素组分（PCF）的体内和体外心血管活性。口服给予高糖高血压大鼠10 mg/kg PCF剂量可降低动脉收缩压和心率，静脉注射相同剂量的N-G-硝基-L-精氨酸甲酯（L-NAME 31 mg/kg）可明显降低血压。在这些实验中，我们采用卡巴胆碱作为阳性对照试验。PCF减少去甲肾上腺素（1 × 10（-7）M）诱导的正常血压（IC 50 = 35.3 +/- 12.4 ng/mL）和高糖高血压（IC 50 = 101.3 +/- 57.2 ng/mL）大鼠离体主动脉环收缩。这种舒张活性抑制血管内皮细胞的去除或L-NAME（30 μ M）预处理，而吲哚美辛（10 μ M）或阿托品（10 μ M）没有影响。PCF的HPLC-DAD-MS和FAB（+）质谱法的初步分析允许检测的主要成分，如复合物的原花青素低聚物主要由四聚体和trimers.Conclusions组成：Guazuma ulmifolia树皮具有持久的抗高血压和血管舒张性能与内皮相关的因素，其中一氧化氮参与。(C)2008爱思唯尔爱尔兰有限公司保留所有权利。
The aim of the study: was to investigate the in vivo and in vitro cardiovascular activity of a procyanidin fraction (PCF) obtained from acetone extract of Guazuma ulmifolia bark which has traditionally been used as an antihypertensive agent.Results: 10 mg/kg PCF doses orally administered to sugar-fed hypertensive rats decreased both the systolic arterial pressure and the heart rate, whereas the same doses intravenously administered induced arterial hypotension which was attenuated by N-G-nitro-L-arginine methylester (L-NAME 31 mg/kg) pretreatment. In these experiments we employed carbachol as a positive control test. The PCF reduced the contraction induced by norepinephrine (1 x 10(-7) M) in isolated aortic rings of normotensive (IC50 = 35.3 +/- 12.4 ng/mL) and sugar-fed hypertensive (IC50 = 101.3 +/- 57.2 ng/mL) rats. This relaxant activity was inhibited by either vascular endothelium removal or L-NAME (30 mu M) pretreatment, while indomethacin (10 mu M) or atropine (10 mu M) had no effect. Preliminary analysis of the PCF by HPLC-DAD-MS and FAB(+) mass spectrometry allowed the detection of the main components such as the complex of procyanidin oligomers consisting mainly of tetramers and trimers.Conclusions: Guazuma ulmifolia bark possesses long-lasting antihypertensive and vasorelaxing properties linked to the endothelium related factors, where nitric oxide is involved. (C) 2008 Elsevier Ireland Ltd. All rights reserved.