Construction of an infectious full-length cDNA clone of Chrysanthemum virus B
Construction of an infectious full-length cDNA clone of Chrysanthemum virus B
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DOI：
10.1007/s10327-008-0120-6
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发表时间：
2008-09
影响因子：
1.2
通讯作者：
A. Ohkawa;Noriko Ishikawa-Suehiro;S. Okuda;T. Natsuaki
中科院分区：
文献类型：
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作者：
A. Ohkawa;Noriko Ishikawa-Suehiro;S. Okuda;T. Natsuaki
构建了菊花病毒B（CVB，卡拉病毒属）的感染性全长cDNA克隆。覆盖CVB-S全基因组的四个cDNA片段被克隆在花椰菜花叶病毒35S启动子和胭脂碱合酶(NOS)终止子之间。使用基因枪系统将构建的质粒（命名为pCVB）接种到菊花和花环菊花上。与野生型 CVB 感染植物的情况一样，在接种 pCVB 的植物上没有观察到明显的症状；然而，蛋白质印迹和电子显微镜表明存在 pCVB 子代病毒。 pCVB 可能是分析卡拉病毒蛋白功能的有用工具。
An infectious full-length cDNA clone ofChrysanthemum virus B(CVB, genusCarlavirus), was constructed. Four cDNA fragments covering the whole genome of CVB-S were cloned between theCauliflower mosaic virus35S promoter and the nopaline synthase (NOS) terminator. Chrysanthemum and garland chrysanthemum were inoculated with the constructed plasmid, named pCVB, using a gene gun system. As is the case in wild-type, CVB-infected plants, no visible symptoms were observed on plants inoculated with pCVB; however, western blotting and electron microscopy indicated the presence of the progeny virus of pCVB. pCVB could be a useful tool for analyzing the functions of carlaviral proteins.