Recombinant mouse gamma interferon induces the priming step in macrophage activation for tumor cell killing.
Recombinant mouse gamma interferon induces the priming step in macrophage activation for tumor cell killing.
复制标题
DOI：
10.4049/jimmunol.130.5.2011
复制
发表时间：
1983-05
影响因子：
4.4
通讯作者：
J. Pace;S. Russell;B. Torres;H. Johnson;P. Gray
中科院分区：
文献类型：
--
作者：
J. Pace;S. Russell;B. Torres;H. Johnson;P. Gray
小鼠巨噬细胞通过一系列步骤（1-3）被激活以杀死肿瘤细胞。其中第一种不会引起细胞溶解活性的表达;相反，它引发巨噬细胞对第二种信号作出反应，然后触发杀伤的开始（4-7）。伴刀豆球蛋白A刺激的脾细胞（5-7）、克隆的T淋巴细胞（8）或一些T细胞杂交瘤（9）的培养上清液中含有负责引发的介质，沿着其他淋巴因子。已经注意到巨噬细胞引发活性与γ干扰素的抗病毒活性之间的密切关联（10-12）。然而，没有明确的证据表明这两种活性是同一分子的产物。我们现在如何决定性地通过使用小鼠γ干扰素（MulFN-γ）3产生的重组DNA技术，除了抗病毒活性，这种淋巴因子有能力诱导的启动步骤的过程中的巨噬细胞激活肿瘤细胞的杀伤。
Mouse macrophages become activated to kill tumor cells by traversing a series of steps (1-3). The first of these does not cause the expression of cytolytic activity; instead, it primes macrophages to respond to a second signal(s) that then triggers the onset of killing (4-7). The mediator that is responsible for priming is contained, along with other lymphokines, in the culture supernatants of concanavalin A-stimulated spleen cells (5-7) cloned T lymphocytes (8), or some T cell hybridomas (9). Close association has been noted between macrophage priming activity and antiviral activity that is attributable to gamma interferon (10-12). However, unequivocal evidence that the two activities are products of the same molecule has not been available. We now how conclusively by using mouse gamma interferon (MulFN-gamma)3 produced by recombinant DNA technology that, in addition to antiviral activity, this lymphokine has the capacity to induce the priming step in the process of macrophage activation for tumor cell killing.