Synchrony dynamics during initiation, failure, and rescue of the segmentation clock
Synchrony dynamics during initiation, failure, and rescue of the segmentation clock
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DOI：
10.1126/science.1142538
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发表时间：
2007-09-28
期刊：
影响因子：
56.9
通讯作者：
Oates, Andrew C.
中科院分区：
文献类型：
--
作者：
Riedel-Kruse, Ingmar H.;Mueller, Claudia;Oates, Andrew C.
“分割时钟”被认为是协调脊椎动物胚胎中体轴的顺序分割。该时钟包括同步振荡器的多细胞遗传网络，通过细胞间Delta-Notch信号传导耦合。这种同步性是如何建立的，以及它的丢失如何决定Delta和Notch突变体中分割缺陷的位置，目前还不清楚。我们通过改变斑马鱼胚胎中Notch偶联的强度和时间，利用基因功能定量扰动技术分析了时钟的同步动力学。我们开发了一个物理理论的基础上耦合相位振荡器解释所观察到的发病和抢救的分割缺陷，时钟的鲁棒性对发展的噪音，和一个临界点，超过同步衰减。我们的结论是，这些遗传振荡器之间的同步可以建立同时启动和自组织和分割缺陷的位置是由耦合强度和噪声之间的差异。
The "segmentation clock" is thought to coordinate sequential segmentation of the body axis in vertebrate embryos. This clock comprises a multicellular genetic network of synchronized oscillators, coupled by intercellular Delta-Notch signaling. How this synchrony is established and how its loss determines the position of segmentation defects in Delta and Notch mutants are unknown. We analyzed the clock's synchrony dynamics by varying strength and timing of Notch coupling in zebrafish embryos with techniques for quantitative perturbation of gene function. We developed a physical theory based on coupled phase oscillators explaining the observed onset and rescue of segmentation defects, the clock's robustness against developmental noise, and a critical point beyond which synchrony decays. We conclude that synchrony among these genetic oscillators can be established by simultaneous initiation and self-organization and that the segmentation defect position is determined by the difference between coupling strength and noise.