Bone regeneration after topical BMP-2-gene delivery in circumferential peri-implant bone defects
Bone regeneration after topical BMP-2-gene delivery in circumferential peri-implant bone defects
复制标题
DOI：
10.1111/j.1600-0501.2007.01526.x
复制
发表时间：
2008-06-01
影响因子：
4.3
通讯作者：
Schlegel, Karl Andreas
中科院分区：
文献类型：
--
作者：
Lutz, Rainer;Park, Jung;Schlegel, Karl Andreas
目的：评价携带骨形态发生蛋白-2(BMP-2)基因的脂质体系统联合胶原载体和自体骨作为载体的局部基因治疗新鲜种植体周围骨缺损后的骨形成和骨整合情况。材料和方法：8头家猪每头造成9个颅骨缺损区(10×7 mm)。在每个缺损处的中心都植入了一颗种植体。试验组用脂质体/骨形态发生蛋白-2复合体复合胶原载体(n=18)或自体骨移植(n=18)填充剩余空隙。结果：术后7d和28d，BMP-2/骨移植组矿化率(29.9%+/-4.8和68.3%+/-7.2)与单纯骨移植组(22.6%+/-2.6%和49.4%+/-13.9)相比差异有统计学意义。28天后，BMP-2/胶原组的矿化值(21.2%+/-16.2和53.1%+/-12.5)也显著高于单纯胶原组(8.2%+/-7和41%+/-8.1)。与对照组相比，BMP-2/骨移植28d组、BMP-2/胶原组7d和28d骨-种植体接触明显增加。结论：BMP-2/BMP-2脂质体/BMP-2对种植体周围骨缺损区的骨再生和骨整合有明显的促进作用。
Objectives: The aims of this study were to evaluate the rate of bone formation and osseointegration after topical gene delivery with a liposomal vector system carrying bone morphogenetic protein (BMP)-2 cDNA in combination with a collagen carrier and autologous bone as a carrier in freshly created peri-implant bone defects.Materials and methods: Eight domestic pigs received nine calvariae defects each (10 x 7 mm). A dental implant was inserted into the centre of each defect. In the test groups, the remaining space was filled with the liposomal vector/BMP-2 complex combined with a collagen carrier (n=18) or an autologous bone graft (n=18). Control groups were collagen only (n=18) and autologous bone graft only (n=18).Results: There was a significant difference in mineralisation rate in the BMP-2/bone graft (29.9% +/- 4.8 and 68.3% +/- 7.2) and bone graft only (22.6% +/- 2.6 and 49.4% +/- 13.9) groups after 7 and 28 days. Mineralisation values were also significantly higher in the BMP-2/collagen group (21.2% +/- 16.2 and 53.1% +/- 12.5) compared with the collagen-only group (8.2% +/- 7 and 41% +/- 8.1) in two different regions after 28 days. Also the bone-to-implant contact was significantly increased in the BMP-2/bone graft group after 28 days and in the BMP-2/collagen group after 7 and 28 days compared with their control groups.Conclusions: The results of this study show a significantly positive effect of liposomal vector/BMP-2 on bone regeneration and osseointegration in bony circumferential peri-implant defects.