Bioelectronic nose for methyl mercaptan vapor using xenobiotic metabolizing enzyme: flavin-containing monooxygenase
Bioelectronic nose for methyl mercaptan vapor using xenobiotic metabolizing enzyme: flavin-containing monooxygenase
复制标题
DOI：
10.1016/s0925-4005(01)01025-5
复制
发表时间：
2002-03-15
影响因子：
8.4
通讯作者：
Hashimoto, Y
中科院分区：
文献类型：
--
作者：
Mitsubayashi, K;Hashimoto, Y
利用黄素单加氧酶（FMO）构建了恶臭物质之一甲基苯丙胺（MM）的酶电极和生物电子鼻。FMO固定化电极的特性被成功地改善，通过应用与抗坏血酸（阿萨）作为还原剂的底物再生循环，从而获得对MM溶液从0.03到4.98 mmol/l，与10.0 mmol/l的阿萨的校准曲线。生物鼻也通过将FMO 3固定化电极结合到具有由多孔疏水膜分隔的气体和液体隔室的反应池中来制造。该装置可以监测1.0至4000 ppm的气相中的MM，具有由FMO 3底物特异性引起的高气体选择性。作为比较的结果，几种MM传感器与异构体。FMOs，FMO 3（3型）固定鼻适用于MM蒸汽的测量，因为其输出电流高于其他设备。(C)2002 Elsevier Science B. V.保留所有权利。
An enzyme electrode and a bioelectronic nose (bio-nose) for methyl mercaptan (MM), as one of the malodorous substances were constructed using flavin-containing monooxygenase (FMO). The characteristics of the FMO immobilized electrodes were successfully improved by applying a substrate regeneration cycle with ascorbic acid (AsA) as reducing reagent, thus obtaining the calibration curve against MM solutions from 0.03 to 4.98 mmol/l, with 10.0 mmol/l AsA. The bio-nose was also fabricated by incorporating the FMO3 immobilized electrode into a reaction cell with both gas and liquid compartments separated by a porous hydrophobic membrane. The device was possible to monitor MM in the gas phase from 1.0 to 4000 ppm, with high gas selectivity caused by the FMO3 substrate specificity. As the results of comparison between several kinds of the MM sensors with isoform. FMOs, the FMO3 (type 3) immobilized nose was suitable for the measurement of MM vapor because of higher output current than those of other devices. (C) 2002 Elsevier Science B.V. All rights reserved.