Distribution of Langerhans cells and mast cells within the human oral mucosa: new application sites of allergens in sublingual immunotherapy?
Distribution of Langerhans cells and mast cells within the human oral mucosa: new application sites of allergens in sublingual immunotherapy?
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DOI：
10.1111/j.1398-9995.2007.01611.x
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发表时间：
2008-06-01
期刊：
影响因子：
12.4
通讯作者：
Novak, N.
中科院分区：
文献类型：
--
作者：
Allam, J. -P.;Stojanovski, G.;Novak, N.
背景：舌下免疫治疗（SLIT）是皮下免疫治疗的替代方案。虽然抗原呈递细胞如朗格汉斯细胞（LC）被认为有助于SLIT的有效性，但肥大细胞（MC）最有可能导致不良反应，如舌下水肿。由于鲜为人知的是LC和MCs在口腔内，我们研究了它们的分布在寻找粘膜网站最高的LC和最低的MCs density.Methods：活检同时从人类前庭，颊，腭，舌，舌下，牙龈，和皮肤。采用免疫组化和流式细胞术检测MC、LC及其高亲和力IgE受体（Fc epsilon RI）的表达。混合淋巴细胞反应进行评估其刺激capacity.Results：最高密度的MCs检测牙龈内，而最低密度的MCs被发现在腭和舌。然而，舌下MC位于腺体内，这可能解释了一些SLIT患者的舌下肉阜肿胀。前庭区LC密度最高，舌下区最低。在前庭内的LC上检测到最高的Fc ε RI表达。此外，来自不同地区的LC显示类似的刺激能力对同种异体T cells.Conclusions：鉴于我们的数据，不同的粘膜区域，如前庭可能代表替代SLIT应用网站与强有力的过敏原摄取。我们的数据可能作为SLIT的新的应用策略，以提高效率和减少局部不良反应的基础。
Background: Sublingual immunotherapy (SLIT) represents an alternative to subcutaneous immunotherapy. While antigen-presenting cells such as Langerhans cells (LCs) are thought to contribute to the effectiveness of SLIT, mast cells (MCs) most likely account for adverse reactions such as sublingual edema. As little is known about LCs and MCs within the oral cavity, we investigated their distribution in search for mucosal sites with highest LCs and lowest MCs density.Methods: Biopsies were taken simultaneously from human vestibulum, bucca, palatum, lingua, sublingua, gingiva, and skin. Immunohistochemistry and flow cytometry were used to detect MCs, LCs and high affinity receptor for IgE (Fc epsilon RI) expression of LCs. Mixed lymphocyte reactions were performed to assess their stimulatory capacity.Results: Highest density of MCs was detected within the gingiva, while the lowest density of MCs was found within the palatum and lingua. However, sublingual MCs were located within glands, which might explain swelling of sublingual caruncle in some SLIT patients. Highest density of LCs was detected within the vestibular region with lowest density in sublingual region. Highest expression of Fc epsilon RI was detected on LCs within the vestibulum. Furthermore LCs from different regions displayed similar stimulatory capacity towards allogeneic T cells.Conclusions: In view of our data, different mucosal regions such as the vestibulum might represent alternative SLIT application sites with potent allergen uptake. Our data might serve as a basis for new application strategies for SLIT to enhance efficiency and reduce local adverse reactions.