Extracting genomic DNA of foodstuff by polyamidoamine (PAMAM)-magnetite nanoparticles.
Extracting genomic DNA of foodstuff by polyamidoamine (PAMAM)-magnetite nanoparticles.
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DOI：
10.1016/j.talanta.2012.02.007
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发表时间：
2012-05
期刊：
影响因子：
6.1
通讯作者：
Fengxiang Qie;Guoxin Zhang;J. Hou;Xiaoming Sun;S. Luo;T. Tan
中科院分区：
文献类型：
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作者：
Fengxiang Qie;Guoxin Zhang;J. Hou;Xiaoming Sun;S. Luo;T. Tan
虽然转基因食品越来越多，但消费者越来越意识到需要识别转基因和非转基因食品：因此，可靠地识别转基因/非转基因食品是社会、健康和安全辩论中的一个重要工具。目前的研究响应这一需求（i）通过开发一种新的“单锅”制备PAMAM磁铁矿纳米颗粒（PMNPs），并充分定义其特定的特性;（ii）通过证明PMNPs的能力，从不同的样品食品中分离基因组DNA;和（iii）通过实验证明通过凝胶电泳鉴定分离的DNA，从而可以对转基因和非转基因食品进行筛选。
Although genetically modified (GM) food is becoming increasingly available, consumers are showing a growing awareness about the need to identify GM and non-GM foodstuff: the reliable identification of GM/non-GM food is therefore an important tool in the social, health and safety debates. The present research responds to this need (i) through developing a novel “single-pot” preparation of PAMAM magnetite nanoparticles (PMNPs) and by fully defining their specific characteristics; (ii) by demonstrating the capability of the PMNPs to isolate genomic DNA from different sample foods; and (iii) by experimentally demonstrating the identification of the isolated DNA by gel-electrophoresis, thus being capable of screening GM and non-GM food.