STUDIES ON INTER-CELLULAR LETS GLYCOPROTEIN MATRICES
STUDIES ON INTER-CELLULAR LETS GLYCOPROTEIN MATRICES
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DOI：
10.1016/0092-8674(78)90123-x
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发表时间：
1978-01-01
期刊：
影响因子：
64.5
通讯作者：
WALSH, ML
中科院分区：
文献类型：
--
作者：
CHEN, LB;MURRAY, A;WALSH, ML
用扫描电镜观察了培养的鸡胚成纤维细胞分泌的细胞间基质。细胞-细胞接触是表达这种基质的先决条件。透射电子显微镜检测到的最小纤维直径为5-10 nm。这些基质纤维倾向于聚集成束。免疫荧光和免疫铁蛋白程序显示，LETS [大外部转化敏感]蛋白是基质的成分之一。通过去污剂处理将基质与其他细胞器分离。无细胞基质中超过90%的蛋白质是LETS蛋白，这表明基质可能仅由LETS蛋白组成。由于基质的增溶需要β-巯基乙醇，LETS蛋白质基质可能是自然界中第一个已知的使用二硫键作为分子间聚合媒介物的聚合物系统。胶原蛋白似乎不参与这种基质。LETS蛋白基质支持圆形细胞向纺锤形的形态转化，并且还促进成肌细胞融合。它不会对细胞生长、葡萄糖摄取速率或蛋白酶产生影响。
Intercellular matrices secreted by chick embryo fibroblasts in culture were studied by scanning electron microscopy. Cell-cell contact is a prerequisite for the expression of such matrices. The smallest fiber detected by transmission electron microscopy is 5-10 nm in diameter. These matrix fibers tend to cluster to form bundles. Immunofluorescence and immunoferritin procedures reveal that LETS [large external transformation-sensitive] protein is one of the components of the matrices. The matrices are isolated from other cellular organelles by detergent treatment. More than 90% of the proteins in cell-free matrices are LETS protein, suggesting that the matrices are probably made of only 1 component, LETS protein. Since the solubilization of matrices requires .beta.-mercaptoethanol, LETS protein matrices may be the first known polymer system in nature to use disulfide linkage as an intermolecular polymerization vehicle. Collagen does not appear to be involved in such matrices. The LETS protein matrix supports the morphological conversion of rounded cells into spindle-shaped, and also promotes myoblast fusion. It does not exert an effect on cell growth, the rate of glucose uptake or protease production.