Isobaric Tagging for Relative and Absolute Protein Quantification (iTRAQ)-Based Quantitative Proteomics Analysis of Differentially Expressed Proteins 1 Week After Spinal Cord Injury in a Rat Model.
Isobaric Tagging for Relative and Absolute Protein Quantification (iTRAQ)-Based Quantitative Proteomics Analysis of Differentially Expressed Proteins 1 Week After Spinal Cord Injury in a Rat Model.
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大鼠模型脊髓损伤后1周差异表达蛋白质的相对和绝对蛋白质定量（iTRAQ）定量蛋白质组学分析的等压标记。
DOI：
10.12659/msm.924266
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发表时间：
2020-11-04
期刊：
影响因子：
--
通讯作者：
Zhou H
中科院分区：
文献类型：
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作者：
Liu S;Kang Y;Zhang C;Lou Y;Li X;Lu L;Qi Z;Jian H;Zhou H
脊髓损伤(SCI)是中枢神经系统(CNS)的一种破坏性损伤，发病率、致残率和死亡率都很高。脊髓损伤后一周可能是治疗的关键时间。蛋白表达的变化在神经系统疾病中具有关键作用，尽管脊髓损伤后1周发生的变化对患者预后的影响尚不清楚。在大鼠脊髓挫伤模型中检测脊髓损伤后1周的蛋白表达。采用等压标记技术进行相对和绝对蛋白质定量(ITRAQ)-液质联用(LC-MS/MS)蛋白质组学分析。基因本体论(GO)分析用于鉴定已鉴定的DEPS的生物学过程、分子功能和细胞组成项，京都基因和基因组百科全书(KEGG)用于鉴定关键的丰富途径。分析蛋白质-蛋白质相互作用(PPI)网络以确定前10个高度核心蛋白质。在295个DEP中，204个(69.15%)在伤后1周上调，91个(30.85%)下调。已确定的主要细胞成分、分子功能、生物学过程和通路可能是脊髓损伤的关键机制。前10个高度核心蛋白是补体成分C3(C3)、α-2-HS-糖蛋白(Ahsg)、T-激肽原1(Kng1)、Serpinc1蛋白(Serpinc1)、载脂蛋白A-I(ApoA1)、血清白蛋白(Alb)、二硫键异构酶蛋白(P4hb)、转运蛋白Sec61亚单位α异构体1(Sec61a1)、转铁蛋白(Tf)和60S核糖体蛋白L15(Rpl15)。本研究鉴定的蛋白质可能为脊髓损伤后1周的诊断和治疗提供潜在的靶点。
Spinal cord injury (SCI) is a devastating trauma of the central nervous system (CNS), with high levels of morbidity, disability, and mortality. One week after SCI may be a critical time for treatment. Changes in protein expression have crucial functions in nervous system diseases, although the effects of changes occurring 1 week after SCI on patient outcomes are unclear. Protein expression was examined in a rat contusive SCI model 1 week after SCI. Differentially expressed proteins (DEPs) were identified by isobaric tagging for relative and absolute protein quantification (iTRAQ)-coupled liquid chromatography tandem-mass spectrometry (LC-MS/MS) proteomics analysis. Gene Ontology (GO) analysis was performed to identify the biological processes, molecular functions, and cellular component terms of the identified DEPs, and the Kyoto Encyclopedia of Genes and Genomes (KEGG) was used to identify key enriched pathways. Protein–protein interaction (PPI) networks were analyzed to identify the top 10 high-degree core proteins. Of the 295 DEPs identified, 204 (69.15%) were upregulated and 91 (30.85%) were downregulated 1 week after injury. The main cellular components, molecular functions, biological processes, and pathways identified may be crucial mechanisms involved in SCI. The top 10 high-degree core proteins were complement component C3 (C3), alpha-2-HS-glycoprotein (Ahsg), T-kininogen 1 (Kng1), Serpinc1 protein (Serpinc1), apolipoprotein A-I (Apoa1), serum albumin (Alb), disulfide-isomerase protein (P4hb), transport protein Sec61 subunit alpha isoform 1 (Sec61a1), serotransferrin (Tf), and 60S ribosomal protein L15 (Rpl15). The proteins identified in this study may provide potential targets for diagnosis and treatment 1 week after SCI.