Dynein and MEL-28 contribute in parallel to oogenic maturity in C. elegans.
Dynein and MEL-28 contribute in parallel to oogenic maturity in C. elegans.
复制标题
动力蛋白和 MEL-28 共同促进秀丽隐杆线虫卵子的成熟。
DOI：
10.17912/micropub.biology.000421
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发表时间：
2021
影响因子：
--
通讯作者：
Fernandez AG
中科院分区：
文献类型：
--
作者：
Gandhi J;Crosio G;Fernandez AG
DHC-1（OR 283 TS）; Mel-28（T1684）双突变体与野生型相比和与每种单突变体相比具有严重降低的窝数。为了确定这种低生育力表型是否与卵母细胞成熟缺陷相关，我们使用标记物来评估近端性腺中卵母细胞的成熟度。我们研究了磷酸化组蛋白H3，一种通常与成熟卵母细胞相关的标记物，和DAO-5，一种通常与未成熟卵母细胞相关的核仁标记物。我们发现，在双突变体中，占据-1位置的卵母细胞经常保留DAO-5，并且无法积累磷酸化组蛋白H3。这表明动力蛋白和MEL-28的同时破坏可导致卵母细胞成熟程序的失败。
dhc-1(or283ts); mel-28(t1684) double mutants have a severely reduced brood size compared to the wild-type and compared to each single mutant. To determine if this low-fecundity phenotype is associated with oocyte maturity defects, we used markers to assess the maturity of oocytes in the proximal gonad. We studied phosphorylated histone H3, a marker normally associated with mature oocytes, and DAO-5, a nucleolar marker normally associated with immature oocytes. We found that in the double mutants, the oocyte occupying the -1 position frequently retains DAO-5 and fails to accumulate phosphorylated histone H3. This suggests that the simultaneous disruption of dynein and MEL-28 can lead to failure of the oocyte maturity program.