IDENTIFICATION AND SEQUENCE DETERMINATION OF THE HOST FACTOR GENE FOR BACTERIOPHAGE-Q-BETA
IDENTIFICATION AND SEQUENCE DETERMINATION OF THE HOST FACTOR GENE FOR BACTERIOPHAGE-Q-BETA
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DOI：
10.1093/nar/19.5.1063
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发表时间：
1991-03-11
影响因子：
14.9
通讯作者：
ISHIHAMA, A
中科院分区：
文献类型：
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作者：
KAJITANI, M;ISHIHAMA, A
从噬菌体感染的大肠杆菌细胞中纯化出噬菌体q - β RNA定向合成互补负链RNA所需的宿主因子（HF-I）。利用基于HF-I部分氨基酸序列设计的合成探针克隆了编码HF-I的hfq基因，并在94.8 min定位于参与2-甲基硫代- n6 -（异戊基）-腺苷（ms2i6A） tRNA修饰的miaA基因下游的大肠杆菌染色体上。克隆的hfq基因序列测定表明，该基因是Mr为11166的小蛋白，由102个氨基酸残基组成。
The host factor (HF-I) required for phage Q-beta RNA-directed synthesis of complementary minus-strand RNA was purified to homogeneity from phage-infected Escherichia coli cells. The hfq gene encoding HF-I was cloned using synthetic probes designed based on the partial amino acid sequence of HF-I, and mapped at 94.8 min on the E. coli chromosome downstream of the miaA gene involved in 2-methylthio-N6-(isopentyl)-adenosine (ms2i6A) tRNA modification. Sequence determination of the cloned hfq gene indicated that HF-I is a small protein of Mr 11,166 consisting of 102 amino acid residues.