Fabrication of individual alginate-TCP scaffolds for bone tissue engineering by means of powder printing
Fabrication of individual alginate-TCP scaffolds for bone tissue engineering by means of powder printing
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DOI：
10.1088/1758-5090/7/1/015004
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发表时间：
2015-03-01
期刊：
影响因子：
9
通讯作者：
Vorndran, Elke
中科院分区：
文献类型：
--
作者：
Castilho, Miguel;Rodrigues, Jorge;Vorndran, Elke
开发具有定制结构和性能的聚合物-磷酸钙复合支架具有巨大的骨再生潜力。在此，我们旨在通过开发一种有前途的生物聚合物-陶瓷网络来改善脆性陶瓷支架的功能性能。为此，跟踪了两种策略，即，直接打印由α/β-磷酸三钙（TCP）粉末和藻酸盐粉末的60：40混合物组成的粉末组合物，或用藻酸盐溶液真空渗透打印的TCP支架。结构表征的结果表明，与2.5重量%的海藻酸盐改性的TCP粉末打印的支架呈现出均匀分布和相互融合的海藻酸盐TCP网络。机械结果表明，一个显着增加的强度，能量失效和可靠性的粉末改性支架与藻酸盐含量的离析物中的2.5重量%相比，纯TCP，以及TCP支架含有5重量%或7.5重量%的离析物，在干燥和潮湿状态。在这些支架上培养人成骨细胞也显示出细胞增殖和细胞活力的极大改善。而在粉末混合藻酸盐TCP支架的情况下，磷酸钙晶体之间形成了孤立的藻酸盐凝胶，真空渗透策略导致TCP支架的表面和内部孔隙被一层薄藻酸盐膜覆盖。此外，在更复杂的应力状态下的支架的临界断裂条件的预测所应用的莫尔断裂准则证实了与2.5重量%的藻酸盐在离析物中的粉末改性的支架作为骨组织工程的结构生物材料的潜力。
The development of polymer-calcium phosphate composite scaffolds with tailored architectures and properties has great potential for bone regeneration. Herein, we aimed to improve the functional performance of brittle ceramic scaffolds by developing a promising biopolymer-ceramic network. For this purpose, two strategies, namely, direct printing of a powder composition consisting of a 60: 40 mixture of alpha/beta-tricalcium phosphate (TCP) powder and alginate powder or vacuum infiltration of printed TCP scaffolds with an alginate solution, were tracked. Results of structural characterization revealed that the scaffolds printed with 2.5 wt% alginate-modified TCP powders presented a uniformly distributed and interfusing alginate TCP network. Mechanical results indicated a significant increase in strength, energy to failure and reliability of powder-modified scaffolds with an alginate content in the educts of 2.5 wt% when compared to pure TCP, as well as to TCP scaffolds containing 5 wt% or 7.5 wt% in the educts, in both dry and wet states. Culture of human osteoblast cells on these scaffolds also demonstrated a great improvement of cell proliferation and cell viability. While in the case of powder-mixed alginate TCP scaffolds, isolated alginate gels were formed between the calcium phosphate crystals, the vacuum-infiltration strategy resulted in the covering of the surface and internal pores of the TCP scaffold with a thin alginate film. Furthermore, the prediction of the scaffolds' critical fracture conditions under more complex stress states by the applied Mohr fracture criterion confirmed the potential of the powder-modified scaffolds with 2.5 wt% alginate in the educts as structural biomaterial for bone tissue engineering.