Canonical heat shock element in the αB-crystallin gene shows tissue-specific and developmentally controlled interactions with heat shock factor
Canonical heat shock element in the αB-crystallin gene shows tissue-specific and developmentally controlled interactions with heat shock factor
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DOI：
10.1074/jbc.m000304200
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发表时间：
2000-06-02
影响因子：
4.8
通讯作者：
Bhat, SP
中科院分区：
文献类型：
--
作者：
Somasundaram, T;Bhat, SP
热休克因子(HSF)的寡聚化及其与热休克元件(HSE)的相互作用是对有形的物理或化学胁迫做出主动转录反应的标志。目前尚不清楚这些相互作用是否受到发育线索的控制和调节，从而具有组织或阶段特异性。通过使用包含来自αB-晶体蛋白基因的典型HSE的启动子序列，我们证明了在出生后10-21天，眼晶状体中从单体(在胎儿和早期新生儿阶段，缺乏寡聚体HSF.HSE复合体)到寡聚体HSF-HSE相互作用的组织特异性转变。这些相互作用的发育控制进一步通过体外操作诱导新生儿提取物中的低聚HSF.HSE复合体来证明，有趣的是，仅在晶状体中，而不在脑、心脏或肝脏提取物中。在出生后/成人晶状体中独有的低聚HSF-HSE复合体的存在与已知该组织中αB-晶状体蛋白转录本的高度增加相对应。
Oligomerization of the heat shock factor (HSF) and its interaction with the heat shock element (HSE) are the hallmark of active transcriptional response to tangible physical or chemical stress. It is unknown if these interactions are subject to control and modulation by developmental cues and thus have tissue or stage specificity. By using promoter sequences containing a canonical HSE from the alpha B-crystallin gene, we demonstrate a tissue-specific transition from monomeric (in fetal and early neonatal stages that lack oligomeric HSF.HSE complexes) to oligomeric HSF-HSE interactions by postnatal day 10-21 in the ocular lens. Developmental control of these interactions is further demonstrated by induction of oligomeric HSF.HSE complexes in neonatal extracts by in vitro manipulations, interestingly, only in the lens and not in the brain, heart, or liver extracts. The exclusive presence of oligomeric HSF HSE complexes in the postnatal/adult lens corresponds to known highly increased number of alpha B-crystallin transcripts in this tissue.