A mouse model for postoperative fatal enteritis due to Staphylococcus infection.
A mouse model for postoperative fatal enteritis due to Staphylococcus infection.
复制标题
葡萄球菌感染引起的术后致死性肠炎小鼠模型。
DOI：
10.1006/jsre.2000.6043
复制
发表时间：
2001
期刊：
影响因子：
--
通讯作者：
T. Kakiuchi
中科院分区：
文献类型：
--
作者：
Yoichi Nakamura;Y. Aramaki;T. Kakiuchi
背景
术后肠道感染耐甲氧西林金黄色葡萄球菌(MRSA)在某些情况下是致命的。本研究的目的是建立一种小鼠感染模型，为研究感染进展的机制提供有用的工具。
方法
用环磷酰胺对小鼠进行预处理，将术后患者制备的MRSA直接或口服注入空肠，然后每天给予5剂抗生素。注射后48h检测细菌移位和血清内毒素水平。在注射MRSA前4d，用脂质体包裹的二氯亚甲基二磷酸(Cl2)MDP去除巨噬细胞。
结果
空肠注射但不口服MRSA可引起肠炎和腹泻，并导致大多数环磷酰胺治疗的小鼠死亡。观察到MRSA在肠系膜淋巴结和肝脏中的移位，并伴有大肠杆菌感染。感染MRSA的内毒素抵抗C3H/HeJ小鼠存活时间长于内毒素敏感的C3H/HeJ小鼠，但也在注射MRSA后一周内死亡。选择性去除巨噬细胞可诱导未经环磷酰胺预处理的小鼠感染。
结论
我们建立了致命性MRSA感染的小鼠模型，该模型可导致腹泻的肠炎，这将是研究术后患者肠道有时致命性MRSA感染的机制的有用工具。在MRSA诱导的肠炎早期，大肠杆菌或内毒素的存在似乎是导致小鼠死亡的主要原因，但在后期，其他因素可能来自MRSA。吞噬细胞对MRSA感染具有重要的保护作用。
BACKGROUND
Postoperative infection of intestine with methicillin-resistant Staphylococcus aureus (MRSA) is fatal in some cases. The object of this study was to establish a mouse model for the infection, providing a useful tool for investigating mechanisms in the progression of infection.
METHODS
Mice were pretreated with cyclophosphamide, injected orally or directly into jejunum with MRSA prepared from a postoperative patient, and then given 5 daily doses of antibiotics. Forty-eight hours after the injection, bacterial translocation and serum endotoxin levels were examined. Macrophage depletion was carried out by the administration of liposome-encapsulated dichloromethylene diphosphate (Cl(2)MDP), 4 days before MRSA injection.
RESULTS
Injection into the jejunum but not oral administration of MRSA induced enteritis with diarrhea and resulted in death in most cyclophosphamide-treated mice. Translocation of MRSA in mesenteric lymph nodes and liver was observed, concomitantly with E. coli infection. Endotoxin-resistant C3H/HeJ mice infected with MRSA survived longer than endotoxin-sensitive C3H/He mice, but also died within a week after MRSA injection. Selective depletion of macrophages induced infection in mice that were not pretreated with cyclophosphamide.
CONCLUSION
We established a mouse model for the fatal MRSA infection which induced enteritis with diarrhea, that will be a useful tool for investigating the mechanisms for sometimes fatal MRSA infection of the intestine in postoperative patients. The presence of E. coli or endotoxin seemed to play a major role in the mortality of mice in the early days of MRSA-induced enteritis, but other factors, probably from MRSA, in the later days. Phagocytes were quite important for protection against the MRSA infection.
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影响因子：
3
作者：
A. Sori;B. Rush;T. Lysz;Sharon Smith;G. Machiedo
通讯作者：
A. Sori;B. Rush;T. Lysz;Sharon Smith;G. Machiedo
影响因子：
--
作者：
E. Deitch;J. Winterton;R. Berg
通讯作者：
E. Deitch;J. Winterton;R. Berg
脂质体介导的巨噬细胞耗竭对 LPS 诱导的细胞因子基因表达和辐射防护的影响。
DOI：
--
发表时间：
1995
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Salkowski,CA;Neta,R;Wynn,TA;Strassmann,G;vanRooijen,N;Vogel,SN
通讯作者：
Vogel,SN
用内毒素中和抗体治疗中毒性休克综合征。
DOI：
10.1016/0022-4804(89)90014-0
发表时间：
1989
期刊：
The Journal of surgical research
影响因子：
--
作者：
Priest,BP;Schlievert,PM;Dunn,DL
通讯作者：
Dunn,DL