Chronic hyperbaric oxygen treatment elicits an anti-oxidant response and attenuates atherosclerosis in apoE knockout mice
Chronic hyperbaric oxygen treatment elicits an anti-oxidant response and attenuates atherosclerosis in apoE knockout mice
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DOI：
10.1016/j.atherosclerosis.2006.08.018
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发表时间：
2007-07-01
期刊：
影响因子：
5.3
通讯作者：
Dory, Ladislav
中科院分区：
文献类型：
--
作者：
Kudchodkar, Bhalchandra J.;Pierce, Anson;Dory, Ladislav
我们先前证明，高压氧（HBO）治疗抑制了新西兰白兔子饮食诱导的动脉粥样硬化。在本研究中，我们研究了HBO治疗的动脉粥样硬化效应可能涉及的机制，该动脉粥样硬化模型是APOE敲除（KO）小鼠。我们检查了每日HBO治疗（5周和10周）对抗氧化剂防御机制和氧化还原状态在血液，肝脏和主动脉组织中的影响，并将其与未经治疗的APOE KO小鼠进行比较。 HBO治疗可显着降低主动脉胆固醇含量和脂肪条纹形成的降低。这些变化伴随着对氧化修饰的LDL的自身抗体的显着降低，以及肝脏和主动脉组织的氧化还原状态的深刻变化。 10周的治疗可显着降低肝脏水平的TBAR和氧化谷胱甘肽，而显着增加了谷胱甘肽，谷胱甘肽还原酶（GR），转移酶，SE依赖性谷胱甘肽过氧化物酶和过氧化酶（CAT）的降低水平。 HBO治疗的作用在主动脉组织中相似。这些观察结果证明了HBO治疗对相关组织的氧化还原状态具有强大的影响，并产生抑制氧化的环境。抗氧化剂反应可能是HBO治疗的抗动脉粥样硬化作用的关键。 （c）2006年Elsevier Ireland Ltd.保留所有权利。
We previously demonstrated that hyperbaric oxygen (HBO) treatment inhibits diet-induced atherosclerosis in New Zealand White rabbits. In the present study we investigate the mechanisms that might be involved in the athero-protective effect of HBO treatment in a well-accepted model of atherosclerosis, the apoE knockout (KO) mouse. We examine the effects of daily HBO treatment (for 5 and 10 weeks) on the components of the anti-oxidant defense mechanism and the redox state in blood, liver and aortic tissues and compare them to those of untreated apoE KO mice. HBO treatment results in a significant reduction of aortic cholesterol content and decreased fatty streak formation. These changes are accompanied by a significant reduction of autoantibodies against oxidatively modified LDL and profound changes in the redox state of the liver and aortic tissues. A 10-week treatment significantly reduces hepatic levels of TBARS and oxidized glutathione, while significantly increases the levels of reduced glutathione, glutathione reductase (GR), transferase, Se-dependent glutathione peroxidase and catalase (CAT). The effects of HBO treatment are similar in the aortic tissues. These observations provide evidence that HBO treatment has a powerful effect on the redox state of relevant tissues and produces an environment that inhibits oxidation. The anti-oxidant response may be the key to the anti-atherogenic effect of HBO treatment. (c) 2006 Elsevier Ireland Ltd. All rights reserved.