cDNA microarray analysis of differential gene expression in Candida albicans biofilm exposed to farnesol
cDNA microarray analysis of differential gene expression in Candida albicans biofilm exposed to farnesol
复制标题
法呢醇暴露的白色念珠菌生物膜差异基因表达的cDNA微阵列分析
DOI：
10.1128/aac.49.2.584-589.2005
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发表时间：
2005-02-01
影响因子：
4.9
通讯作者：
Jiang, YY
中科院分区：
文献类型：
--
作者：
Cao, YY;Cao, YB;Jiang, YY
白色念珠菌生物膜是具有高水平耐药性的结构化微生物群落。法尼醇是一种群体感应分子，可抑制C.白色念珠菌，已经发现可以防止由C.白色念珠菌然而，关于法尼醇对抗生物膜形成的分子机制的信息有限。我们使用cDNA微阵列分析来鉴定一个C.法尼醇抑制白色念珠菌生物膜。共聚焦扫描激光显微镜用于可视化和确认正常和法尼醇抑制的生物膜。共有274个基因被鉴定为应答基因，其中104个基因上调，170个基因下调。使用独立的逆转录-PCR分析来确认通过微阵列分析检测到的重要变化。除了菌丝形成相关基因（例如，TUP 1、CRK 1和PDE 2），许多其他与耐药性相关的基因（例如，FCR 1和PDR 16）、细胞壁维持（例如，CHT 2和CHT 3）和铁转运（例如，FTR 2）是响应性的，编码热休克蛋白的几个基因（例如，HSP 70、HSP 90、HSP 104、CaMSI 3和SSA2）。这些差异调节基因的进一步研究是必要的，以评估它们如何参与C。白色念珠菌生物膜形成。与细胞表面疏水性相关基因（CSH 1）的下调一致，水-烃两相测定显示，与对照组相比，法尼醇处理组的细胞表面疏水性降低。我们的研究结果为进一步了解法尼醇抗念珠菌的分子机制提供了新的思路。白色念珠菌生物膜形成。
Candida albicans biofilms are structured microbial communities with high levels of drug resistance. Farnesol, a quorum-sensing molecule that inhibits hyphal formation in C. albicans, has been found to prevent biofilm formation by C. albicans. There is limited information, however, about the molecular mechanism of farnesol against biofilm formation. We used cDNA microarray analysis to identify the changes in the gene expression profile of a C. albicans biofilm inhibited by farnesol. Confocal scanning laser microscopy was used to visualize and confirm normal and farnesol-inhibited biofilms. A total of 274 genes were identified as responsive, with 104 genes up-regulated and 170 genes down-regulated. Independent reverse transcription-PCR analysis was used to confirm the important changes detected by microarray analysis. In addition to hyphal formation-associated genes (e.g., TUP1, CRK1, and PDE2), a number of other genes with roles related to drug resistance (e.g., FCR1 and PDR16), cell wall maintenance (e.g., CHT2 and CHT3), and iron transport (e.g., FTR2) were responsive, as were several genes encoding heat shock proteins (e.g., HSP70, HSP90, HSP104, CaMSI3, and SSA2). Further study of these differentially regulated genes is warranted to evaluate how they may be involved in C. albicans biofilm formation. Consistent with the down-regulation of the cell surface hydrophobicity-associated gene (CSH1), the water-hydrocarbon two-phase assay showed a decrease in cell surface hydrophobicity in the farnesol-treated group compared to that in the control group. Our data provide new insight into the molecular mechanism of farnesol against C. albicans biofilm formation.