THE MECHANISM OF ADHESION OF CELLS TO GLASS. A STUDY BY INTERFERENCE REFLECTION MICROSCOPY.
THE MECHANISM OF ADHESION OF CELLS TO GLASS. A STUDY BY INTERFERENCE REFLECTION MICROSCOPY.
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DOI：
10.1083/jcb.20.2.199
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发表时间：
1964-02
期刊：
影响因子：
--
通讯作者：
CURTIS AS
中科院分区：
文献类型：
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作者：
CURTIS AS
一种测量薄膜厚度的光学技术已被改编和评估，用于研究组织培养中细胞与玻璃的粘附结构。这种技术，这是所谓的干涉反射显微镜，已被用来研究鸡胚心脏成纤维细胞。观察到这些发现：在正常培养基中，细胞表面在其粘附中与基质最接近的距离约为。100 A，大部分细胞表面位于更远的地方;使细胞表面接近其电荷反转点的化学处理将粘附的最近接近度降低至<50 A，可能降低至<30 A;增加表面电荷的化学处理增加了粘附中细胞和基底的最近接近度，从约100 A到约100 A。100 A;非极性物质（即蔗糖）的高渗透浓度不影响粘附中细胞与基质之间的距离。此外，光学证据表明，在粘附的细胞和玻璃之间没有细胞外物质。当细胞从玻璃上脱落时，它们似乎不会留下碎片。这些成纤维细胞中的粘附位点似乎局限于细胞面向基质的一侧的边缘和伪足。这一点的意义进行了讨论的接触抑制的现象。有证据表明，细胞粘附的机制并不涉及钙原子结合细胞基质上的羧基结合细胞表面，基板，并与水泥物质。四氧化锇固定导致细胞和基质之间的最终分离为100至200埃：有理由认为这种与生活条件相当接近的方法是作为人工制品产生的。这些结果只能用静电斥力和引力的作用来解释，引力可能是货车德瓦尔斯-伦敦力。生物学论据表明，这些结果同样适用于细胞间粘附。
An optical technique for measuring the thickness of thin films has been adapted and evaluated for studying the structure of the adhesion of cells to glass in tissue culture. This technique, which is termed interference reflection microscopy, has been used to study embryonic chick heart fibroblasts. These findings have been observed: in normal culture medium the closest approach of the cell surface to substrate in its adhesions is ca. 100 A, much of the cell surface lying farther away; chemical treatments which bring the cell surface to near its charge reversal point reduce the closest approach of adhesions to <50 A, probably to <30 A; chemical treatments which increase surface charge increase the nearest approach of cell and substrate in adhesions from ca. 100 A; high osmotic concentration of a non-polar substance, i.e. sucrose, does not affect the distance between cell and substrate in the adhesions. In addition, optical evidence indicates that there is no extracellular material between cell and glass in the adhesions. When cells de-adhere from glass, they appear not to leave fragments behind. The adhesive sites in these fibroblasts appear to be confined to the edge of the side of the cell facing the substrate and to the pseudopods. The significance of this is discussed in relation to the phenomenon of contact inhibition. Evidence is presented that the mechanism of cell adhesion does not involve calcium atoms binding cells to substrate by combining with carboxyl groups on cell surface, substrate, and with a cement substance. Osmium tetroxide fixation results in a final separation of 100 to 200 A between cell and substrate: there are reasons for thinking that this fairly close approach to the condition in life is produced as an artefact. The results can be accounted for only in terms of the action of electrostatic repulsive forces and an attractive force, probably the van der Waals—London forces. Biological arguments suggest that these results are equally applicable for cell-to-cell adhesions.