Molecular structure of the human albumin gene is revealed by nucleotide sequence within q11-22 of chromosome 4.
Molecular structure of the human albumin gene is revealed by nucleotide sequence within q11-22 of chromosome 4.
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DOI：
10.1016/s0021-9258(19)62680-3
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发表时间：
1986-05
期刊：
影响因子：
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通讯作者：
P. P. Minghetti-P.;D. E. Ruffner;W. Kuang;O. Dennison;J. Hawkins;W. Beattie;A. Dugaiczyk
中科院分区：
文献类型：
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作者：
P. P. Minghetti-P.;D. E. Ruffner;W. Kuang;O. Dennison;J. Hawkins;W. Beattie;A. Dugaiczyk
人白蛋白基因从推定的“帽”位点到第一个poly（A）添加位点跨越16，961个核苷酸。它被14个插入序列分成15个外显子，这些插入序列对称地位于白蛋白的三个结构域内。5'区高度保守至-250位，并含有推定的TATA（-32）和CAT（-88）盒。共有5'剪接序列读作/GTAGAGT，而3'剪接序列富含嘧啶并在剪接点处含有CTAG/。该基因含有三个多聚腺苷酸化信号，并且该3'区域可能是由哺乳动物辐射前较短片段的三倍化产生的。白蛋白基因表现出高度的DNA多态性，似乎最近已被入侵的Alu重复序列。
The human albumin gene spans 16,961 nucleotides from the putative “Cap” site to the first poly(A) addition site. It is split into 15 exons by 14 intervening sequences which are symmetrically placed within the three domains of albumin. The 5' region is highly conserved up to position -250 and contains the putative TATA (-32) and CAT (-88) boxes. A consensus 5' splice sequence reads /GTAGAGT while the 3' splice sequence is pyrimidine rich and contains CTAG/ at the splice junction. The gene contains three polyadenylation signals, and this 3' region presumably arose by triplication of a shorter fragment prior to mammalian radiation. The albumin gene exhibits a high degree of DNA polymorphism and appears to have been recently invaded by Alu repetitive sequences.