Resilience of the dominant human fecal microbiota upon short-course antibiotic challenge
Resilience of the dominant human fecal microbiota upon short-course antibiotic challenge
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DOI：
10.1128/jcm.43.11.5588-5592.2005
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发表时间：
2005-11-01
影响因子：
9.4
通讯作者：
Doré, J
中科院分区：
文献类型：
--
作者：
De La Cochetière, MF;Durand, T;Doré, J
最近的研究表明，人类粪便微生物群由对宿主具有特异性的物种组成，并且随着时间的推移对修饰具有抗性。已知抗生素会影响肠道微生物菌群，随后的变化可能导致腹泻。因此，重要的是要描述这些变化的性质和幅度，以及这个生态系统恢复到其原始轮廓的能力，即，它的韧性。6名健康志愿者口服阿莫西林（1.5 g/天）5天。在抗生素治疗前第0天（DO）以及此后60天内的设定时间间隔收集粪便样品。分离粪便DNA，用通用引物PCR扩增16 S rRNA基因的V6-至-V8区域，并通过时间温度梯度凝胶电泳分析。根据相似性（皮尔逊相关系数）比较优势种概况。DO的优势物种概况用作参考。粪便微生物群在抗生素治疗后显示出优势物种的重大转变，从治疗开始后24小时开始，4天后达到平均相似性仅为74%。在抗生素治疗后30天内，粪便微生物群倾向于达到与DO值的平均相似性为88%;在60天内，与DO值的平均相似性为89%。然而，在一名受试者中，重要的修改持续了至少2个月，与DO值的相似性保持在70%以下。我们证明了占主导地位的人类粪便微生物群对短程抗生素挑战的适应性。然而，在某些受试者中持续存在的长期变化可能解释了与腹泻相关的易感性。此外，这些发现表明，加强粪便微生物群抵抗修饰的能力的策略将具有临床意义。
Recent studies have shown that the human fecal microbiota is composed of a consortium of species specific to the host and resistant to modifications over time. Antibiotics are known to affect the intestinal microflora, and ensuing changes may result in antibiotic-associated diarrhea. It is therefore important to characterize the nature and amplitude of these modifications and the ability of this ecosystem to return to its original profile-i.e., its resilience. Six healthy volunteers received oral amoxicillin (1.5 g/day) for 5 days. Fecal samples were collected at day 0 (DO) before antibiotic treatment and at set intervals until 60 days thereafter. Fecal DNA was isolated, and V6-to-V8 regions of the 16S rRNA genes were amplified by PCR with general primers and analyzed by temporal temperature gradient gel electrophoresis. Dominant species profiles were compared on the basis of similarity (Pearson correlation coefficient). Dominant species profiles at DO were used as a reference. The fecal microbiota showed a major shift in dominant species upon antibiotic treatment, starting 24 h after treatment initiation and reaching an average similarity of only 74% after 4 days. Within 30 days following antibiotic treatment, the fecal microbiota tended to reach an average similarity of 88% to the DO value; within 60 days, the average similarity to the DO value was 89%. However, in one subject, important modifications persisted for at least 2 months, with similarity to the DO value remaining below 70%. We demonstrated the resilience of the dominant human fecal microbiota upon short-course antibiotic challenge. Yet the persistence of long-term alterations in some subjects may explain susceptibilities to antibiotic-associated diarrhea. Furthermore, these findings suggest that strategies reinforcing the ability of the fecal microbiota to resist modifications would be of clinical relevance.