Asymmetric Mach-Zehnder Interferometer Based Biosensors for Aflatoxin M1 Detection.
Asymmetric Mach-Zehnder Interferometer Based Biosensors for Aflatoxin M1 Detection.
复制标题
DOI：
10.3390/bios6010001
复制
发表时间：
2016-01-06
期刊：
影响因子：
--
通讯作者：
Pavesi L
中科院分区：
文献类型：
--
作者：
Chalyan T;Guider R;Pasquardini L;Zanetti M;Falke F;Schreuder E;Heideman RG;Pederzolli C;Pavesi L
本文研究了基于Si 3 N4非对称马赫-曾德尔干涉仪（aMZI）和抗体片段（Fab′）的光子生物传感器对黄曲霉毒素M1的检测。我们测得的最佳体积灵敏度为104 rad/RIU，导致检测限低于5 × 10−7 RIU。在用Fab′功能化的传感器上，我们在各种毒素浓度下进行了特异性和非特异性传感测量。测量的再现性和传感器的可重复使用性也进行了研究。
In this work, we present a study of Aflatoxin M1 detection by photonic biosensors based on Si3N4 Asymmetric Mach–Zehnder Interferometer (aMZI) functionalized with antibodies fragments (Fab′). We measured a best volumetric sensitivity of 104 rad/RIU, leading to a Limit of Detection below 5 × 10−7 RIU. On sensors functionalized with Fab′, we performed specific and non-specific sensing measurements at various toxin concentrations. Reproducibility of the measurements and re-usability of the sensor were also investigated.