PREPARATION AND CHARACTERIZATION OF A VIABLE SUSPENSION OF POSTEMBRYONIC HUMAN EPIDERMAL CELLS
PREPARATION AND CHARACTERIZATION OF A VIABLE SUSPENSION OF POSTEMBRYONIC HUMAN EPIDERMAL CELLS
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DOI：
10.1038/jid.1967.26
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发表时间：
1967-01-01
影响因子：
6.5
通讯作者：
WHEELER, CE
中科院分区：
文献类型：
--
作者：
BRIGGAMAN, RA;ABELE, DC;WHEELER, CE
描述了一种制备活的人表皮细胞悬浮液的方法。用胰蛋白酶分离表皮，然后机械地将表皮细胞从分离的整个表皮上移除，制备表皮细胞悬浮液。细胞被进一步胰蛋白酶解离成一个基本的单细胞悬浮液。通过细胞聚集、附着在玻璃上以及在玻璃上形成单层的细胞生长，证明了细胞的生存能力。上皮细胞和黑素细胞在培养中初步鉴定。测定细胞总数和细胞活力。
A method for preparing viable human epidermal cell suspensions is described. An epidermal cell suspension was prepared by tryptic separation of the epidermis followed by the mechanical removal of the epidermal cells from the whole separated epidermis. The cells were dissociated by further trypsinization to an essentially single cell suspension. Viability of the cells was demonstrated in culture by aggregation of cells, attachment to glass and outgrowth of cells forming monolayer sheets on glass. Epithelial cells and melanocytes were tentatively identified in culture. Total cell numbers and cell viability were measured.