Dynamic adsorption properties of n-alkyl glucopyranosides determine their ability to inhibit cytolysis mediated by acoustic cavitation.
Dynamic adsorption properties of n-alkyl glucopyranosides determine their ability to inhibit cytolysis mediated by acoustic cavitation.
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DOI：
10.1021/jp805380e
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发表时间：
2008-10-09
期刊：
影响因子：
--
通讯作者：
Riesz P
中科院分区：
文献类型：
--
作者：
Sostaric JZ;Miyoshi N;Cheng JY;Riesz P
当暴露于高于声空化阈值的超声波时，人类白血病 (HL-60) 细胞的悬浮液很容易发生细胞溶解。然而，正烷基吡喃葡萄糖苷（己基、庚基和辛基）完全抑制超声诱导的 (1057 kHz) 细胞溶解 (Sostaric, et al., Free Radic. Biol. Med. 2005, 39, 1539–1548)。防止超声诱导的细胞溶解的功效由吡喃葡萄糖苷的正烷基链长度决定，表明保护功效取决于正烷基吡喃葡萄糖苷对空化气泡的气体/溶液界面和/或细胞脂质膜的吸附。目前的研究测试了这样的假设：体外“声保护”（即保护细胞免受超声波诱导的细胞溶解）取决于空化气泡气体/溶液界面上吡喃葡萄糖苷的吸附。为了检验这一假设，研究了超声频率（从 42 kHz 至 1 MHz）对一系列同源正烷基吡喃葡萄糖苷保护细胞免受超声诱导的细胞溶解的能力的影响。预计超声波频率将影响声波防护能力，因为空化气泡场的性质将会改变。这将影响超声诱导细胞溶解的可能机制的相对重要性。此外，超声波频率将影响空化气泡的寿命和表面积的变化率，从而动态控制吡喃葡萄糖苷对其表面的吸附。这些数据支持这样的假设：声波防护效率取决于吡喃葡萄糖苷在空化气泡的气体/溶液界面上的吸附能力。
Suspensions of human leukemia (HL-60) cells readily undergo cytolysis when exposed ultrasound above the acoustic cavitation threshold. However, n-alkyl glucopyranosides (hexyl-,heptyl- and octyl-) completely inhibit ultrasound-induced (1057 kHz) cytolysis (Sostaric, et al., Free Radic. Biol. Med. 2005, 39, 1539–1548). The efficacy of protection from ultrasound-induced cytolysis was determined by the n-alkyl chain length of the glucopyranosides, indicating that protection efficacy depended on adsorption of n-alkyl glucopyranosides to the gas/solution interface of cavitation bubbles and/or the lipid membrane of cells. The current study tests the hypothesis that “sonoprotection” (i.e., protection of cells from ultrasound-induced cytolysis) in vitro depends on the adsorption of glucopyranosides at the gas/solution interface of cavitation bubbles. To test this hypothesis, the effect of ultrasound frequency (from 42 kHz to 1 MHz) on the ability of a homologous series of n-alkyl glucopyranosides to protect cells from ultrasound-induced cytolysis was investigated. It is expected that ultrasound frequency will affect sonoprotection ability, since the nature of the cavitation bubble field will change. This will affect the relative importance of the possible mechanisms for ultrasound-induced cytolysis. Additionally, ultrasound frequency will affect the lifetime and the rate of change of the surface area of cavitation bubbles, hence the dynamically controlled adsorption of glucopyranosides to their surface. The data support the hypothesis that sonoprotection efficiency depends on the ability of glucopyranosides to adsorb at the gas/solution interface of cavitation bubbles.
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