New milbemycins from Streptomyces hygroscopicus subsp. aureolacrimosus: fermantation, isolation and strucutre elucidation.
New milbemycins from Streptomyces hygroscopicus subsp. aureolacrimosus: fermantation, isolation and strucutre elucidation.
复制标题
DOI：
10.7164/antibiotics.53.694
复制
发表时间：
2000-07
期刊：
影响因子：
--
通讯作者：
Koichi Nonaka;T. Tsukiyama;Yoshihiro Okamoio;Kazuo Sato;Chieko Kumasaka;Takahiro Yamamoto;F. Maruyama;Hiroji Yoshikawa
中科院分区：
文献类型：
--
作者：
Koichi Nonaka;T. Tsukiyama;Yoshihiro Okamoio;Kazuo Sato;Chieko Kumasaka;Takahiro Yamamoto;F. Maruyama;Hiroji Yoshikawa
从吸湿链霉菌亚种中分离并鉴定了12种新的米尔贝霉素。号沉没60286号和60526号。菌株RM28D-688沉没60797产生的代谢物1约4和9约11为次要产物。从菌株57-338沉没61796的肉汤中获得了大约8个代谢物。菌株MK-1391沉没62896用于代谢物12的生产。新的代谢物，8个α类和4个β类化合物，具有新的结构特征。例如，milbemycins alpha26和alpha27具有26-羟基部分，其他衍生物（milbemycins alpha20约23）在C-26位置具有与milbemycins alpha11和alpha14不同的侧链。此外，还发现了参与25-甲基和25-乙基米尔霉素主要生物合成途径的5-羟基米尔霉素beta7 （beta12）。
Twelve new milbemycins have been isolated and characterized from some strains derived from Streptomyces hygroscopicus subsp. aureolacrimosus SANK 60286 and SANK 60526. The metabolites 1 approximately 4 and 9 approximately 11 were produced by strain RM28D-688 SANK 60797 as minor products. The metabolites 5 approximaetly 8 were obtained from a broth of strain 57-338 SANK 61796. Strain MK-1391 SANK 62896 was used for the production of metabolite 12. The new metabolites, eight alpha-class and four beta-class compounds, have new structural features. For example, milbemycins alpha26 and alpha27, have the 26-hydroxy moiety, and other derivatives (milbemycins alpha20 approximately 23) have different side chains at the C-26 position from those of milbemycins alpha11 and alpha14. In addition, 5-hydroxylmilbemycin beta7 (beta12), involved in the major biosynthetic pathway of 25-methyl and 25-ethyl milbemycins, was discovered.