Induced pluripotent stem cells, new tools for drug discovery and new hope for stem cell therapies.
Induced pluripotent stem cells, new tools for drug discovery and new hope for stem cell therapies.
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DOI：
10.2174/1874467210902010015
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发表时间：
2009-01
影响因子：
2.7
通讯作者：
Shi Y
中科院分区：
文献类型：
--
作者：
Shi Y
体细胞核移植或治疗性克隆为基于干细胞的治疗提供了巨大的希望。然而，治疗性克隆一直面临着伦理和技术上的困难。最近的突破性研究使用四种因素的组合，在不使用胚胎或卵子的情况下将人类体细胞重编程为多能干细胞，导致了干细胞研究的重要革命。使用形态学、细胞表面标志物表达、基因表达谱、表观遗传状态和分化潜能的测定对人诱导多能干细胞和人胚胎干细胞进行比较分析，揭示了这两种多能干细胞类型之间显著程度的相似性。这篇简短的综述总结了这些突破性的研究。重编程的进展将使创建患者特异性干细胞系以研究各种疾病机制成为可能。所创建的细胞模型将为药物发现提供有价值的工具。此外，这种重编程系统提供了巨大的潜力，使定制的患者特异性干细胞治疗的经济可行性。
Somatic cell nuclear transfer or therapeutic cloning has provided great hope for stem cell-based therapies. However therapeutic cloning has been experiencing both ethical and technical difficulties. Recent breakthrough studies using a combination of four factors to reprogram human somatic cells into pluripotent stem cells without using embryos or eggs led to an important revolution in stem cell research. Comparative analysis of human induced pluripotent stem cells and human embryonic stem cells using assays for morphology, cell surface marker expression, gene expression profiling, epigenetic status, and differentiation potential revealed a remarkable degree of similarity between these two pluripotent stem cell types. This mini-review summarizes these ground-breaking studies. The advance in reprogramming will enable the creation of patient-specific stem cell lines to study various disease mechanisms. The created cellular models will provide valuable tools for drug discovery. Furthermore, this reprogramming system provides great potential to make customized patient-specific stem cell therapy with economical feasibility.