Expression of ionotropic glutamate receptor genes by P19 embryonal carcinoma cells.
Expression of ionotropic glutamate receptor genes by P19 embryonal carcinoma cells.
复制标题
P19胚胎癌细胞的离子型谷氨酸受体基因的表达。
DOI：
10.1006/bbrc.1993.2643
复制
发表时间：
1993
影响因子：
3.1
通讯作者：
Gottlieb,DI
中科院分区：
文献类型：
--
作者：
Ray,WJ;Gottlieb,DI
P19 embryonal carcinoma cells can be induced to differentiate into neuron-like cells by retinoic acid. P19 neurons were recently shown to express both NMDA and non-NMDA type glutamate receptor-mediated currents and be susceptible to glutamate excitotoxicity. In this study, we used RT-PCR to survey differentiated P19 cultures for glutamate receptor transcript expression. The following transcripts were detected: at least one member of the GluR1-4 family, GluR5, GluR6, GluR7, KA1, KA2, NMDAR1, and NMDAR2B. Nuclease protection assays revealed a large quantitative induction of GluR6 transcripts following retinoic acid treatment. Ionotropic glutamate receptors are a fundamental and major feature of CNS neurons which are not expressed by the cell lines commonly used as experimental models for mammalian neurons. The present results show that P19 cells express multiple genes involved in glutamate receptor biology. Since the stem cells can be manipulated genetically, the system has the basic requirements for analyzing mechanisms involved in glutamate receptor gene expression.
