STUDIES ON REACTIVITY OF TUMOR AND NORMAL CELLS TO A WHEAT GERM AGGLUTININ
STUDIES ON REACTIVITY OF TUMOR AND NORMAL CELLS TO A WHEAT GERM AGGLUTININ
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DOI：
10.1073/pnas.54.2.396
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发表时间：
1965-01-01
影响因子：
11.1
通讯作者：
COTE, MN
中科院分区：
文献类型：
--
作者：
AUB, JC;SANFORD, BH;COTE, MN
材料和方法：这些研究中使用的小鼠肿瘤是L#2淋巴瘤、6C3HED淋巴瘤和TA3腺癌。1946年，13年，L 2号在A系小鼠体内被辐射诱发，1941.9，6C3HED在C_3H小鼠中被苯甲酸雌二醇诱发。TA3在A系雌性小鼠中自发地出现为乳腺肿瘤。这些肿瘤的2个腹水亚系是通过1)Rloswell Park纪念研究所的T·Hauschka的慷慨获得的。从纽约布法罗Rloswell Park纪念研究所的T·T·Hauschka先生的腹膜腔中取出肿瘤细胞，悬浮在无钙磷酸盐缓冲盐水中，在100x gg下旋转10分钟，然后再悬浮在5%EDTA和PBS中。通过将无菌取出的器官的碎片通过细筛压下，将肺和结缔组织从结缔组织中分离出来，然后将它们包裹并重新悬浮，如上所述。肝细胞用5%/c EDTA原位灌流后按同样方法制备。按Billingham的方法制成表皮和骨髓悬液，6将一只断头小鼠放血，放入含有5%Oedta的试管中，采集红细胞。
Materials and Methods.-The mouse tumors used in these studies were the L# 2 lymphoma, 6C3HED lymphoma, and TA3 adenocarcinoma. The L# 2 was induced in an A-strain mouse by irradiation in 1946, 13 and the 6C3HED in a C3H mouse by estradiol benzoate in 1941.9 The TA3 arose spontaneously as a mammary tumor in an A-strain female.'2 Ascites sublines of these tumors were obtainedthrough the generosity of 1) r. TS Hauschka at Rloswell Park IMemorial Institute, Buffalo, NY Tumor cells were withdrawn from the peritoneal cavity, suspended in calcium-free phosphate-buffered saline (PBS), spun down at 100 X gfor 10 min, and then re-suspended in 5% EDTA and PBS.Cells from normal mouse spleen, thymus, and lung were separated from connective tissue by pressing fragments of aseptically removedorgans through a fine screen, after which they were (entrifilged and resuspended as above. Liver cells were prepared in the same way after perfusion of the liver in situ with 5%/c, EDTA. Epidermal and bone marrow suspensions were made according to Billingham's methods, 6 and erythrocytes were collected by bleeding a decapitated mouse into a tube containing 5% Oedta.