Rearrangement and selection of VH11 in the Ly-1 B cell lineage.
Rearrangement and selection of VH11 in the Ly-1 B cell lineage.
复制标题
DOI：
10.1084/jem.172.1.371
复制
发表时间：
1990-07-01
期刊：
影响因子：
--
通讯作者：
Hardy RR
中科院分区：
文献类型：
--
作者：
Carmack CE;Shinton SA;Hayakawa K;Hardy RR
用于编码抗BrMRBC特异性的主要VH基因之一是重排至JH1的小型VH11家族的一个成员。利用聚合酶链反应（PCR），我们已经确定，无论在何处（脾脏或腹腔），具有VH11重排的B细胞在Ly - 1 B细胞中的频率比在Ly - 1⁻“常规”B细胞中高10 - 20倍。常规B细胞在pre - B细胞和成熟B细胞中利用所有JH基因片段对该基因进行重排的水平相当。相比之下，Ly - 1 B细胞中VH11重排水平的升高仅限于JH1（以及一些JH2），因此似乎是选择的结果。此外，大多数具有VH11重排的腹腔Ly - 1 B细胞处于被抗BrMRBC抗体染色的组分中，可能其表面Ig受体上持续结合有多价天然（可能是自身）抗原。因此，我们认为自身抗原在很大程度上是随着时间推移Ly - 1 B细胞谱系中自身抗体特异性积累的原因，而它们在常规B细胞中不产生这种作用。
One of the predominant VH genes utilized to encode the anti-BrMRBC specificity is a member of the small VH11 family rearranged to JH1. Using the polymerase chain reaction (PCR) we have determined that the frequency of B cells with a VH11 rearrangement is 10-20 times higher in Ly-1 B than in Ly-1- "conventional" B cells regardless of location (spleen or peritoneal cavity). Conventional B cells rearrange this gene at comparable levels in pre-B cells and in mature B cells utilizing all JH gene segments. In contrast, the increased levels of VH11 rearrangement in Ly-1 B are restricted to JH1 (and some JH2) and therefore appear to be the result of selection. Furthermore, most peritoneal Ly-1 B cells with VH11 rearrangements fall in a fraction stained by anti-BrMRBC antibody, likely bearing multivalent natural (likely self) antigen constitutively bound to their surface Ig receptors. Thus, we suggest that autoantigens are largely responsible for the accumulation of autoantibody specificities in the Ly-1 B cell lineage with time, whereas they do not exert this effect in the conventional B cells.