The orientation of the three haems of the 'in situ' ubiquinol oxidase, cytochrome bd, of Escherichia coli.
The orientation of the three haems of the 'in situ' ubiquinol oxidase, cytochrome bd, of Escherichia coli.
复制标题
大肠杆菌“原位”泛醇氧化酶细胞色素 bd 的三个血红素的方向。
DOI：
10.1042/bj2820255
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Salerno,JC
中科院分区：
文献类型：
--
作者：
Ingledew,WJ;Rothery,RA;Gennis,RB;Salerno,JC
大肠杆菌细胞色素 bd 复合物包含三个血红素作为辅基。在三价铁形式中，主要是高自旋二氢卟酚 (血红素 d)、高自旋血红素 b (b595) 和低自旋血红素 b (b558)。这三个血红素的方向已由 e.p.r 确定。细胞质膜片段定向多层制备的研究。低自旋血红素 b (b558) 和高自旋血红素 d 的血红素平面垂直于膜平面。高自旋血红素 b595 的血红素平面定向为大约。与膜平面成 55 度。次要的低自旋分量归因于血红素 d 的低自旋亚群，其血红素平面也垂直于膜平面。
The Escherichia coli cytochrome bd complex incorporates three haems as prosthetic groups. In the ferric form these are a predominantly high-spin chlorin (haem d), a high-spin haem b (b595) and a low-spin haem b (b558). The orientations of these three haems have been determined by e.p.r. studies on oriented multilayer preparations of cytoplasmic membrane fragments. The low-spin haem b (b558) and the high-spin haem d are oriented with their haem planes perpendicular to the membrane plane. The high-spin haem b595 is oriented with its haem plane at approx. 55 degrees to the membrane plane. A minor low-spin component, attributable to a low-spin subpopulation of the haem d, is also oriented with its haem plane perpendicular to the membrane plane.