Characterization of glycoconjugates of human gastrointestinal mucosa by lectins. II. Lectin binding to the isolated glycoproteins of normal and malignant gastric mucosa.
Characterization of glycoconjugates of human gastrointestinal mucosa by lectins. II. Lectin binding to the isolated glycoproteins of normal and malignant gastric mucosa.
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凝集素对人胃肠粘膜糖缀合物的表征。
DOI：
10.1177/32.7.6429236
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
R. Fischer
中科院分区：
文献类型：
--
作者：
J. Fischer;G. Uhlenbruck;P. Klein;M. Vierbuchen;R. Fischer
用HPA-、PNA-、Con A和WGA-琼脂糖柱亲和层析，仅能从Triton X-100溶解的正常和癌变胃粘膜组分中分离出部分（10-30%）高分子量粘液糖蛋白。粘液的主要部分不被凝集素结合，这与我们早期在石蜡包埋组织切片上的凝集素组织化学观察结果一致。凝集素结合的粘液糖蛋白具有相对较低的分子量，范围为约250- 1，000千道尔顿，如聚丙烯酰胺梯度凝胶电泳和Biogel A 1.5 m柱上的凝胶过滤所示。在气相色谱分析中，氨基己糖与半乳糖的摩尔比在凝集素结合的粘液物质中比在整个高分子量粘液中高得多（3：1）。这一发现表明凝集素对粘液中富含己糖胺的组分具有更高的亲和力，这些组分可能是粘液糖蛋白分子的特殊形式或粘液寡糖链的不完全糖基化的核心和主链区域。胃腺癌粘液PNA分离物中氨基己糖含量极高（10：1）。由于已知PNA与位于粘液寡糖链还原端的末端二糖β-半乳糖-（1-3）-N-乙酰半乳糖胺结合，因此极有可能胃癌细胞中寡糖侧链的延长受到干扰。
Using affinity chromatography on HPA-, PNA-, Con A, and WGA-agarose columns only a part (10-30%) of the high molecular weight mucous glycoproteins could be isolated from the Triton X-100 solubilized components of normal as well as carcinomatous gastric mucosa. The main part of the mucus was not bound by the lectins, which corresponds to our earlier lectin histochemical observations on paraffin-embedded tissue sections. The lectin-bound mucous glycoproteins had a relatively lower molecular weight, ranging from about 250-1,000 kilodaltons, as indicated by polyacrylamide gradient gel electrophoresis and by gel filtration on Biogel A 1.5 m column. In gas chromatographic analysis the molar ratio of aminohexoses to galactose was found to be much higher (3:1) in the lectin-bound mucous substances than in the whole high molecular weight mucus (1:1). This finding indicates that lectins have a higher affinity to the hexosamine rich components of mucus, which may be special forms of mucous glycoprotein molecules or the incompletely glycosylated core and backbone regions of the oligosaccharide chains of mucus. Extremely high hexosamine values (10:1) were found in the PNA isolated mucus of gastric adenocarcinoma. Since it is known that PNA binds to the terminal disaccharide, beta-galactose-(1-3)-N-acetylgalactosamine, which is localized at the reducing end of the oligosaccharide chains of mucus, it is highly probable that the elongation of the oligosaccharide side chains is disturbed in gastric cancer cells.