Tumor diagnosis by intermediate filament typing: a novel tool for surgical pathology.
Tumor diagnosis by intermediate filament typing: a novel tool for surgical pathology.
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通过中间丝分型进行肿瘤诊断：外科病理学的一种新工具。
DOI：
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发表时间：
1983
期刊：
影响因子：
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通讯作者：
K. Weber
中科院分区：
文献类型：
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作者：
M. Osborn;K. Weber
在大多数细胞类型中，中间或10 mm的细丝(IF)是主要的细胞骨架组织，因此，直接或间接地影响细胞质的结构外观。根据正常动物和人类组织中不同IF蛋白的细胞类型特异性表达模式，IF分型可以区分主要的肿瘤组，正如通过传统组织学方法对数百种人类肿瘤进行分类的结果所证明的那样。癌的特征是细胞角蛋白，肌肉细胞的肉瘤是结蛋白，非肌肉肉瘤的特征是波形蛋白，胶质瘤的特征是胶质纤维酸性蛋白。此外，某些起源于交感神经系统的肿瘤，如神经节神经母细胞瘤、嗜铬细胞瘤，以及至少一些神经母细胞瘤，都以神经丝的存在为特征。癌症通常可以通过检查其细胞角蛋白图谱，根据其可能的来源进一步细分。起源细胞的IF型特征似乎不仅存在于原发肿瘤，而且通常也存在于实体转移瘤中。一般来说，肿瘤不会获得额外的IF类型。因此，如果打字在某些情况下可以提供明确和快速的特征，这些情况很难通过传统技术进行诊断。一些有用的例子是儿童的小细胞肿瘤，以及未分化癌和淋巴瘤的区别。如果一些肿瘤的分型已经导致修改或重新考虑最初的光镜诊断。综合结果表明，至少某些癌症以及某些其他类型的肿瘤似乎是由于正常组织中存在的特定和可识别的细胞类型的选择性增殖而产生的。这一过程并不局限于肿瘤材料。如果对马洛里小体进行分型，阿尔茨海默病与某些肌病有关，而羊水细胞提供了更有趣的应用。因此，如果分型应该成为组织学和外科病理学中有价值的新工具。
In most cell types intermediate or 10-mm filaments (IF) are a major cytoskeletal organization and, thus, directly or indirectly influence the structural appearance of the cytoplasm. In line with the cell type-specific expression patterns of different IF proteins in normal animal and human tissue, IF typing distinguishes the major tumor groups, as documented by results with several hundred human tumors classified by conventional histologic methods. Carcinomas are characterized by cytokeratins, sarcomas of muscle cells by desmin, nonmuscle sarcomas by vimentin, and gliomas by glial fibrillary acidic protein. Furthermore, certain tumors originating from the sympathetic nervous system, e.g., ganglioneuroblastoma, pheochromocytoma, and at least some neuroblastomas, are characterized by the presence of neurofilaments. Carcinomas can often be further subdivided with regard to their possible derivation by examining their cytokeratin profiles. The IF type characteristic of the cell of origin seems to be kept not only in the primary tumor but usually also in solid metastases. In general, tumors do not acquire additional IF types. Therefore, IF typing can provide an unambiguous and rapid characterization in certain cases, that are difficult to diagnose by conventional techniques. Some useful examples are the small cell tumors of childhood and the discrimination between undifferentiated carcinoma and lymphoma. IF typing of a few tumors has already led to a revision or reconsideration of the original light microscopic diagnosis. The combined results indicate that at least certain carcinomas, as well as certain other tumor types, seem to arise by the selective multiplication of a particular and identifiable cell type present in the normal tissue. The procedure is not restricted to tumor material. IF typing of Mallory bodies, Alzheimer's disease tangles, certain myopathies, and the cells of the amniotic fluid offers further interesting applications. Thus, IF typing should become a valuable new tool both in histology and surgical pathology.