Fast targeted multidimensional NMR metabolomics of colorectal cancer
Fast targeted multidimensional NMR metabolomics of colorectal cancer
复制标题
DOI：
10.1002/mrc.2519
复制
发表时间：
2009-12-01
影响因子：
2
通讯作者：
Guenther, U. L.
中科院分区：
文献类型：
--
作者：
Ludwig, C.;Ward, D. G.;Guenther, U. L.
体液小分子的研究已成为监测生物有机体状态的重要工具。应用范围从使用细胞系的模型研究到使用人体体液来监测疾病状态或药物反应的应用。核磁共振波谱一直是代谢组学的重要工具，尽管信号的严重重叠限制了化合物的数量，这些化合物可以明确地进行识别和量化。因此，核磁共振波谱的去卷积是基于核磁共振的代谢组学的最大挑战之一。这通常是通过使用多维光谱来实现的，多维光谱的缺点是需要显著更长的采集时间。最近，已经描述了许多方法来更快地记录核磁共振谱。在这里，我们探索使用Hadamard编码的TOCSY谱来同时从拥挤的血清样品的核磁共振谱中选择多条线，以在几分钟内获得伪二维谱，否则需要数小时。这种方法的潜力被证明用于从人类血液样本中检测结直肠癌的特征。版权所有(C)2009 John Wiley&Sons，Ltd.
The study of small molecules in body fluids has become an important tool to monitor the state of biological organisms. Applications range from model studies using cell lines to applications where human body fluids are used to monitor disease states or drug responses. NMR spectroscopy has been an important tool for metabolomics although severe overlap of signals has limited the number of compounds, which can be unambiguously identified and quantified. Therefore, deconvolution of NMR spectra is one of the greatest challenges for NMR-based metabolomics. This has commonly been achieved by using multidimensional spectra that have the disadvantage of requiring significantly longer acquisition times. Recently, a number of methods have been described to record NMR spectra much faster. Here, we explore the use of Hadamard-encoded TOCSY spectra to simultaneously select multiple lines from crowded NMR spectra of blood serum samples to acquire pseudo-two-dimensional spectra in minutes which would otherwise require many hours. The potential of this approach is demonstrated for the detection of a signature for colorectal cancer from human blood samples. Copyright (C) 2009 John Wiley & Sons, Ltd.