Cell therapy for spinal cord injury by using human iPSC-derived region-specific neural progenitor cells.
Cell therapy for spinal cord injury by using human iPSC-derived region-specific neural progenitor cells.
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DOI：
10.1186/s13041-020-00662-w
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发表时间：
2020-09-03
期刊：
影响因子：
3.6
通讯作者：
Okano H
中科院分区：
文献类型：
--
作者：
Kajikawa K;Imaizumi K;Shinozaki M;Shibata S;Shindo T;Kitagawa T;Shibata R;Kamata Y;Kojima K;Nagoshi N;Matsumoto M;Nakamura M;Okano H
来源于人诱导多能干细胞（iPSC）的神经祖细胞（NPC）移植对脊髓损伤（SCI）具有有益的作用。然而，虽然存在许多具有不同区域身份的NPC亚型，但尚未确定最适合用于SCI细胞治疗的iPSC衍生的NPC亚型。在这里，我们从人类iPSC中产生了前脑和脊髓型NPC，并将它们移植到小鼠受伤的脊髓上。这两种类型的NPC在移植后保留了它们的区域身份，并表现出不同的移植物-宿主互连特性。具有脊髓区域特性但不具有前脑特性的NPC导致SCI小鼠的功能改善，特别是在那些具有轻度至中度病变的小鼠中。这项研究强调了用于SCI细胞治疗的人iPSC衍生的NPC的区域特性的重要性。
The transplantation of neural progenitor cells (NPCs) derived from human induced pluripotent stem cells (iPSCs) has beneficial effects on spinal cord injury (SCI). However, while there are many subtypes of NPCs with different regional identities, the subtype of iPSC-derived NPCs that is most appropriate for cell therapy for SCI has not been identified. Here, we generated forebrain- and spinal cord-type NPCs from human iPSCs and grafted them onto the injured spinal cord in mice. These two types of NPCs retained their regional identities after transplantation and exhibited different graft-host interconnection properties. NPCs with spinal cord regional identity but not those with forebrain identity resulted in functional improvement in SCI mice, especially in those with mild-to-moderate lesions. This study highlights the importance of the regional identity of human iPSC-derived NPCs used in cell therapy for SCI.
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影响因子：
5.9
作者：
Lippmann, Ethan S.;Williams, Clay E.;Ruhl, David A.;Estevez-Silva, Maria C.;Chapman, Edwin R.;Coon, Joshua J.;Ashton, Randolph S.
通讯作者：
Ashton, Randolph S.
影响因子：
3.4
作者：
Imaizumi K;Fujimori K;Ishii S;Otomo A;Hosoi Y;Miyajima H;Warita H;Aoki M;Hadano S;Akamatsu W;Okano H
通讯作者：
Okano H
影响因子：
5.9
作者：
Fujimori K;Matsumoto T;Kisa F;Hattori N;Okano H;Akamatsu W
通讯作者：
Akamatsu W
影响因子：
2.7
作者：
Okada, Y;Shimazaki, T;Okano, H
通讯作者：
Okano, H
影响因子：
3.7
作者：
Kobayashi Y;Okada Y;Itakura G;Iwai H;Nishimura S;Yasuda A;Nori S;Hikishima K;Konomi T;Fujiyoshi K;Tsuji O;Toyama Y;Yamanaka S;Nakamura M;Okano H
通讯作者：
Okano H