Lipoteichoic acids from Lactobacillus strains elicit strong tumor necrosis factor alpha-inducing activitives in macrophages through toll-like receptor 2
Lipoteichoic acids from Lactobacillus strains elicit strong tumor necrosis factor alpha-inducing activitives in macrophages through toll-like receptor 2
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DOI：
10.1128/cdli.10.2.259-266.2003
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发表时间：
2003-03-01
期刊：
影响因子：
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通讯作者：
Yoshikai, Y
中科院分区：
文献类型：
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作者：
Matsuguchi, T;Takagi, A;Yoshikai, Y
乳酸杆菌是微生物群落中的非致病性革兰氏阳性居民。至少有一些乳酸菌菌株被认为具有健康有益的效果，如刺激免疫系统。在这里，我们研究了乳酸杆菌对小鼠免疫细胞的刺激作用。所有六种热灭活的乳杆菌菌株都能诱导小鼠脾单核细胞分泌肿瘤坏死因子α（TNF-α），尽管程度不同。当在小鼠巨噬细胞系RAW 264.7中测试干酪乳杆菌的分级分离的亚细胞级分的NF-κ B活化和TNF-α产生时，发现活性如下：原生质体>细胞壁远大于多糖-肽聚糖复合物。对两株乳杆菌L.干酪乳杆菌和发酵乳杆菌显著诱导C57 BL/6、C3H/HeN和C3H/Hej小鼠的RAW264.7细胞和脾细胞分泌TNF-α，但不诱导C57 BL/6 TLR2（-/-）小鼠的脾细胞分泌TNF-α。乳杆菌LTA诱导RAW264.7细胞中c-Jun N-末端激酶活化的活化。此外，在用CD14和Toll样受体2（TLR2）的组合横切的HEK293 T细胞中，NF-κ B响应于乳杆菌LTA而被激活。总之，这些数据表明，乳酸杆菌的LTA通过TLR 2引发促炎活性。
Lactobacilli are nonpathogenic gram-positive inhabitants of microflora. At least some Lactobacillus strains have been postulated to have health beneficial effects, such as the stimulation of the immune system. Here we examined the stimulatory effects of lactobacilli on mouse immune cells. All six heat-killed Lactobacillus strains examined induced the secretion of tumor necrosis factor alpha (TNF-alpha) from mouse splenic mononuclear cells, albeit to various degrees. When fractionated subcellular fractions of Lactobacillus casei were tested for NF-kappaB activation and TNF-alpha production in RAW264.7, a mouse macrophage cell line, the activity was found to be as follows: protoplast > cell wall much greater than polysaccharide-peptidoglycan complex.. Both crude extracts and purified lipoteichoic acids (LTAs) from two Lactobacillus strains, L. casei and L.fermentum, significantly induced TNF-alpha secretion from RAW264.7 cells and splenocytes of C57BL/6, C3H/HeN, and C3H/Hej mice but not from splenocytes of C57BL/6 TLR2(-/-) mice. Lactobacillus LTA induced activation of c-Jun N-terminal kinase activation in RAW264.7 cells. Furthermore, in HEK293T cells transected with a combination of CD14 and Toll-like receptor 2 (TLR2), NF-kappaB was activated in response to Lactobacillus LTA. Taken together, these data suggest that LTAs from lactobacilli elicit proinflammatory activities through TLR2.