Protective efficacy of an H5/H7 trivalent inactivated vaccine produced from Re-11, Re-12, and H7-Re2 strains against challenge with different H5 and H7 viruses in chickens
Protective efficacy of an H5/H7 trivalent inactivated vaccine produced from Re-11, Re-12, and H7-Re2 strains against challenge with different H5 and H7 viruses in chickens
复制标题
Re-11、Re-12 和 H7-Re2 株生产的 H5/H7 三价灭活疫苗对鸡不同 H5 和 H7 病毒攻击的保护功效
DOI：
10.1016/s2095-3119(20)63301-9
复制
发表时间：
2020-09-01
影响因子：
4.8
通讯作者：
Chen Hua-lan
中科院分区：
文献类型：
--
作者：
Zeng Xian-ying;Chen Xiao-han;Chen Hua-lan
我们利用反向遗传学产生的Re-11、Re-12和H7-Re 2疫苗种子病毒，并从A/duck/Guizhou/S4184/2017（H5 N6）中获得其HA基因，开发了H5/H7三价灭活疫苗。（DK/GZ/S4184/17）（a clade 2.3.4.4d virus），A/chicken/Liaoning/SD007/2017（H5N1）（CK/LN/SD 007/17）（2.3.2.1d进化枝病毒）和A/chicken/ Guangxi/SD 098/2017（H7N9）（CK/GX/SD 098/17）。在鸡体内评价了该疫苗和最近使用的H5/H7双价灭活疫苗对不同H5和H7N9病毒的保护效力。我们发现，H5/H7双价疫苗对H7N9病毒CK/GX/SD 098/17提供了坚实的保护，但对不同H5病毒的保护率仅为50-60%。相比之下，新的H5/H7三价疫苗提供了对所测试的H5和H7病毒的完全保护。我们的研究强调了及时更新禽流感控制疫苗的重要性。
We developed an H5/H7 trivalent inactivated vaccine by using Re-11, Re-12, and H7-Re2 vaccine seed viruses, which were generated by reverse genetics and derived their HA genes from A/duck/Guizhou/S4184/2017(H5N6) (DK/GZ/S4184/17) (a clade 2.3.4.4d virus), A/chicken/Liaoning/SD007/2017(H5N1) (CK/LN/SD007/17) (a clade 2.3.2.1d virus), and A/chicken/ Guangxi/SD098/2017(H7N9) (CK/GX/SD098/17), respectively. The protective efficacy of this novel vaccine and that of the recently used H5/H7 bivalent inactivated vaccine against different H5 and H7N9 viruses was evaluated in chickens. We found that the H5/H7 bivalent vaccine provided solid protection against the H7N9 virus CK/GX/SD098/17, but only 50-60% protection against different H5 viruses. In contrast, the novel H5/H7 trivalent vaccine provided complete protection against the H5 and H7 viruses tested. Our study underscores the importance of timely updating of vaccines for avian influenza control.