External accumulation of ions for enhanced electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry
External accumulation of ions for enhanced electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry
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DOI：
10.1016/s1044-0305(97)00126-8
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发表时间：
1997-09-01
影响因子：
3.2
通讯作者：
Marshall, AG
中科院分区：
文献类型：
--
作者：
Senko, MW;Hendrickson, CL;Marshall, AG
电喷雾电离（ESI）结合傅立叶变换离子回旋共振（FTICR）质谱法提供了对生物分子的质量分析，具有无与伦比的质量精度、分辨能力和灵敏度。然而，ESI FTICR MS的性能与在线分离技术，如液相色谱（LC）和毛细管电泳迄今已受到限制，主要是由脉冲气体辅助的积累和相关的抽气时间与频繁的离子束采样要求的在线色谱分离的不相容性。在这里，我们描述了大量的分析优势，通过在高压下捕获离子在离子转移到离子阱在磁体的中心，在低压下进行高性能质量分析之前，在双八极离子注入系统的第一个仅射频八极。新的配置提高了连续产生的离子分析的占空比，因此非常适合在线色谱应用。LC/ESI FTICR MS在500 fmol三种肽的混合物上进行证明。其他改进包括信噪比和分辨率比我们仪器上的现有方法提高了五倍。(C)1997年美国质谱学会。
Electrospray ionization (ESI) in combination with Fourier transform ion cyclotron resonance (FTICR) mass spectrometry provides for mass analysis of biological molecules with unrivaled mass accuracy, resolving power and sensitivity. However, ESI FTICR MS performance with on-line separation techniques such as liquid chromatography (LC) and capillary electrophoresis has to date been limited primarily by pulsed gas assisted accumulation and the incompatibility of the associated pump-down time with the frequent ion beam sampling requirement of on-line chromatographic separation. Here we describe numerous analytical advantages that accrue by trapping ions at high pressure in the first rf-only octupole of a dual octupole ion injection system before ion transfer to the ion trap in the center of the magnet for high performance mass analysis at low pressure. The new configuration improves the duty cycle for analysis of continuously generated ions, and is thus ideally suited for on-line chromatographic applications. LC/ESI FTICR MS is demonstrated on a mixture of 500 fmol of each of three peptides. Additional improvements include a fivefold increase in signal-to-noise ratio and resolving power compared to prior methods on our instrument. (C) 1997 American Society for Mass Spectrometry.