Enveloped viruses as model membrane systems: microviscosity of vesicular stomatitis virus and host cell membranes.
Enveloped viruses as model membrane systems: microviscosity of vesicular stomatitis virus and host cell membranes.
复制标题
作为模型膜系统的包膜病毒：水泡性口炎病毒和宿主细胞膜的微粘度。
DOI：
10.1021/bi00661a026
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发表时间：
1976
期刊：
影响因子：
2.9
通讯作者：
R. Wagner
中科院分区：
文献类型：
--
作者：
Y. Barenholz;N. Moore;R. Wagner
采用荧光探针1,6-二苯基-1,3,5-己三烯，研究并比较了水疱性口炎病毒在L-929细胞、两种方法制备的L-929细胞的质膜、病毒脂质和质膜脂质制备的脂质体以及完整的L-929细胞上的疏水区动态特性。在脂质囊泡双层中，探针进入脂质双层疏水区域的速度比完整膜快得多，但在所有情况下，荧光各向异性随时间不变。l细胞的质膜、由质膜衍生的脂质制备的囊泡和完整的细胞在很宽的温度范围内具有比病毒或病毒脂质囊泡低得多的微粘度值。质膜和质膜脂质囊泡的微粘度与质膜制备工艺有关，不同制备方法制备的膜具有不同的微粘度。完整的病毒和由病毒脂质制备的脂质体具有非常相似的微粘度值。质膜和由质膜脂质制备的脂质体也具有相似的微粘度值。讨论了影响天然脂膜和人工混合脂膜微粘度的因素。
The fluorescence probe 1,6-diphenyl-1,3,5-hexatriene was used to study and compare the dynamic properties of the hydrophobic region of vesicular stomatitis virus grown on L-929 cells, plasma membrane of L-929 cells prepared by two different methods, liposomes prepared from virus lipids and plasma membrane lipids, and intact L-929 cells. The rate of penetration of the probe into the hydrophobic region of the lipid bilayer was found to be much faster in the lipid vesicle bilayer as compared with the intact membrane, but in all cases the fluorescence anisotropy was constant with time. The L-cell plasma membranes, the vesicles prepared from the lipids derived from the plasma membranes, and intact cells are found to have much lower microviscosity values than the virus or virus lipid vesicles throughout a wide range of temperatures. The microviscosity of plasma membrane and plasma membrane lipid vesicles was found to depend on the procedure for plasma membrane preparation as the membranes prepared by different methods had different microviscosities. The intact virus and liposomes prepared from the virus lipids were found to have very similar microviscosity values. Plasma membrane and liposomes prepared from plasma membrane lipids also had similar microviscosity values. Factors affecting microviscosity in natural membranes and artificially mixed lipid membranes are discussed.