Comparative analysis of estrogenic activity in sewage treatment plant effluents involving three in vitro assays and chemical analysis of steroids
Comparative analysis of estrogenic activity in sewage treatment plant effluents involving three in vitro assays and chemical analysis of steroids
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DOI：
10.1897/03-286
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发表时间：
2004-04-01
影响因子：
4.1
通讯作者：
Eggen, RIL
中科院分区：
文献类型：
--
作者：
Rutishauser, BV;Pesonen, M;Eggen, RIL
在这项研究中，我们评估和比较了三种体外筛选工具在测量污水处理厂流出物(STPE)中雌激素活性的适用性。这些检测包括酵母雌激素筛选(是)，在鲑鱼肝细胞中产生辐射带蛋白(ZRPs)，以及在转染性腺细胞系RTG-2中诱导报告基因的表达。此外，根据流出物的类固醇分析数据，将YES获得的数据与计算的雌激素活性进行比较。为了比较的目的，体外系统对雌激素化学物质P-雌二醇、乙烯基雌二醇的反应。双酚-A对壬基酚和辛基酚进行了评价。所有三种检测方法对所测试的类固醇化合物的敏感度都是相同的数量级，ZRP产物的敏感度最低。关于测试的雌激素类环境化学物质，RTG-2检测比其他两种检测方法的灵敏度高一个数量级以上。尽管它们对选定的测试化学品的敏感性不同，但这三个体外系统对测试的STPE在相同的浓度范围内显示出雌激素活性。对于被测试的STPE，计算的雌激素活性(化学分析)和测量的雌激素活性(是)具有相同的数量级。本研究表明，三种体外系统都适用于流出物样品中雌激素活性的筛选，其中以酵母为基础的系统最简单和最可靠。
In this study, we assessed and compared the suitability of three in vitro screening tools for the measurement of estrogenic activity in sewage treatment plant effluents (STPEs). These assays were the yeast estrogen screen (YES), production of zona radiata proteins (ZRPs) in trout hepatocytes, and the induction of reporter gene expression in the transfected rainbow trout gonad cell line RTG-2. Data obtained with the YES were additionally compared with calculated estrogenicity, based on steroid analysis data of the effluents. For comparison purposes, the response of the in vitro systems toward the estrogenic chemicals P-estradiol, ethinyl estradiol. bisphenol-A. nonylphenol, and octylphenol was assessed. All three assays showed sensitivities in the same order of magnitude in response to the steroid compounds tested, with ZRP production being the least sensitive. Regarding the estrogenic environmental chemicals tested, the RTG-2 assay was more than an order of magnitude more sensitive than the other two assays. Despite their different sensitivities toward selected test chemicals, the three in vitro systems indicated estrogenic activity in the same concentration range for the tested STPEs. Calculated estrogenicity (chemical analysis) and measured estrogenicity (YES) were of the same order of magnitude for the STPEs tested. The present study indicates that all three in vitro systems, with the yeast-based system being the easiest and most robust, are applicable for the screening of estrogenic activity in effluent samples.