Antibacterial Properties of Nano-Ag Coating on Healing Abutment: An In Vitro and Clinical Study
Antibacterial Properties of Nano-Ag Coating on Healing Abutment: An In Vitro and Clinical Study
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DOI：
10.3390/antibiotics9060347
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发表时间：
2020-06-01
影响因子：
4.8
通讯作者：
Sawase, Takashi
中科院分区：
文献类型：
--
作者：
Odatsu, Tetsurou;Kuroshima, Shinichiro;Sawase, Takashi
种植体周围炎是一种炎症性疾病，与牙种植体和种植体支持的修复体的长期成功相关。本研究主要研究纳米银的抗菌作用，并在体内和体外研究微波辅助纳米银涂层对牙菌斑粘附在种植体基牙和/或上层结构上的抑制作用。采用微波辅助合成方法在纯钛表面制备了纳米银涂层，并用扫描电镜和x射线能谱仪对其进行了表征。体外研究采用MTS法和荧光显微镜分析其与人牙龈成纤维细胞的生物相容性，评价其抗菌活性。在体内研究中，将纳米银涂层应用于愈合基牙，并通过裂口随机临床试验证实纳米银涂层对牙菌斑积累的预防作用。在钛表面发现纳米级颗粒聚集，具有抗菌作用。该涂层对人牙龈成纤维细胞无细胞毒性作用。临床试验结果表明，该涂层能抑制牙菌斑在愈合基面上的粘附。纳米银涂层是一种很有前途的抗菌材料，可用于种植体基台和修复体，预防种植体周围炎。
Peri-implantitis is an inflammatory disease with a relevant focus on the long-term success of dental implants and implant-supported prostheses. The present study focuses on the antibacterial effect of the silver nanoparticle and investigated the suppression of dental plaque adhesion on implant abutment and/or superstructure by micro-wave assistant nanosilver coating in vivo and in vitro. Nanosilver coating on pure titanium was prepared by microwave-assisted synthesis, and characterized by scanning electron microscopy and energy-dispersive X-ray spectroscopy. In vitro studies were conducted to analyze biocompatibility using MTS assay and fluorescence microscopy with human gingival fibroblasts to evaluate antibacterial activity. During the in vivo study, nanosilver coating was applied to the healing abutments, and the prevention of plaque accumulation on nanosilver coating was confirmed by a split-mouth randomized clinical trial. The aggregation of nano-sized particles was found on the titanium surface with an antibacterial effect. The coating had no cytotoxic effect on human gingival fibroblasts. The result of the clinical trial showed that the coating suppressed the dental plaque adhesion on the healing abutments. Nanosilver coating is a promising material with antibacterial properties and can be used for implant abutments and prostheses for preventing peri-implantitis.