Different expression of 25-kDa heat-shock protein (Hsp25) in Meckel's cartilage compared with other cartilages in the mouse
Different expression of 25-kDa heat-shock protein (Hsp25) in Meckel's cartilage compared with other cartilages in the mouse
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DOI：
10.1007/s00429-002-0297-y
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发表时间：
2003-02-01
期刊：
影响因子：
--
通讯作者：
Amano, O
中科院分区：
文献类型：
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作者：
Shimada, M;Yamamoto, M;Amano, O
25 kDa的热休克蛋白（Hsp25）在生长板的软骨中表达，并建议在软骨细胞分化和变性中起作用。使用免疫组织化学，我们研究了时间和空间的发生Hsp25在Meckel的软骨在胚胎小鼠下颌骨，并在其他类型的软骨在胚胎和成年小鼠。在成人中，Hsp25免疫反应性检测位于长骨生长板和非成骨喉和气管软骨中的肥大软骨细胞。没有软骨细胞在静止或增殖期表现出热休克蛋白25免疫反应。在胚胎下颌骨，休息和增殖的软骨细胞在前面和中间部分Meckel的软骨显示Hsp25免疫反应性从妊娠第12天（E12）通过E15，而在后面的部分显示很少或没有免疫反应性。E16后，Meckel软骨中的整体Hsp25免疫反应性的强度显著降低，并且在位于Meckel软骨退化部分的肥大软骨细胞中检测到很少或没有免疫反应性。将Hsp25 mRNA的反义寡核苷酸应用于来自1310个胚胎的下颌骨外植体的培养基中，引起Meckel软骨的前部和中部的发育的显著抑制。这些结果表明，Hsp25是必不可少的Meckel软骨的发展，并发挥不同的作用，在Meckel软骨的永久性软骨和软骨内骨化的软骨。
The 25-kDa heat-shock protein (Hsp25) is expressed in the cartilage of the growth plate and suggested to function in chondrocyte differentiation and degeneration. Using immunohistochemistry, we examined the temporal and spatial occurrence of Hsp25 in Meckel's cartilage in embryonic mice mandibles, and in other types of cartilage in both embryonic and adult mice. In adults, Hsp25 immunoreactivity was detected in the hypertrophic chondrocytes located in growth plates of long bones and in non-osteogenic laryngeal and tracheal cartilages. No chondrocytes in the resting or proliferating phase exhibited Hsp25 immunoreactivity. In the embryonic mandibles, resting and proliferating chondrocytes in the anterior and intermediate portions of Meckel's cartilage showed Hsp25 immunoreactivity from the 12th day of gestation (E12) through E15, whereas those in the posterior portion showed little or no immunoreactivity. After E16, the overall Hsp25 immunoreactivity in Meckel's cartilage substantially reduced in intensity, and little or no immunoreactivity was detected in the hypertrophic chondrocytes located in the degenerating portions of Meckel's cartilage. The antisense oligonucleotide for Hsp25 mRNA applied to the culture media of the mandibular explants from 1310 embryos caused significant inhibition of the development of the anterior and middle portions of Meckel's cartilage. These results suggested that Hsp25 is essential for the development of Meckel's cartilage and plays different roles in Meckel's cartilage from those in the permanent cartilages and the cartilages undergoing endochondral ossification.