Reduced cAMP levels and glycogen phosphorylase activation in isoproterenol perfused SHR myocardium.
Reduced cAMP levels and glycogen phosphorylase activation in isoproterenol perfused SHR myocardium.
复制标题
异丙肾上腺素灌注的 SHR 心肌中 cAMP 水平和糖原磷酸化酶激活降低。
DOI：
10.1007/bf01907217
复制
发表时间：
1983
影响因子：
9.5
通讯作者：
Bhalla,RC
中科院分区：
文献类型：
--
作者：
Sharma,RV;Gupta,RC;Ramanadham,M;Venema,RC;Bhalla,RC
The effect of isoproterenol perfusion on cAMP levels and phosphorylase activity was investigated in the spontaneously hypertensive rat (SHR) and Kyoto Wistar normotensive control rat (WKY) heart. The basal force of contraction in physiological salt solution perfused hearts was comparable between SHR and WKY. However, the force of contraction in response to 10 nM isoproterenol perfusion was decreased approximately 20–30% in SHR heart as compared to WKY heart. Basal cAMP levels were reduced in SHR hearts as compared to WKY heart. Isoproterenol perfusion resulted in an increase in cAMP levels over the basal cAMP values which was 50% and 100% in SHR and WKY hearts, respectively. Basal phosphorylase activity was higher in SHR hearts as compared to WKY hearts. However, the percentage increase in phosphorylase activity by isoproterenol perfusion over the basal values was approximately 400% in WKY hearts and only 200% in SHR hearts. The oubain-sensitive (Na+, K+)-ATPase activity, Ca2+binding in the absence of ATP, sialic acid content, and 5′-nucleotidase activity of purified cardiac plasma membranes was not altered in SHR as compared to WKY. These results would suggest β-adrenergic mediated adenylate cyclase stimulation is decreased in SHR myocardium while other plasma membrane properties and associated enzymes may not be altered.
