Comparison of ultraviolet irradiation-induced mutagenesis of the lacI gene in Escherichia coli and in human 293 cells.
Comparison of ultraviolet irradiation-induced mutagenesis of the lacI gene in Escherichia coli and in human 293 cells.
复制标题
大肠杆菌和人 293 细胞中 lacI 基因紫外线照射诱导突变的比较。
DOI：
10.1016/0022-2836(89)90368-9
复制
发表时间：
1989
影响因子：
5.6
通讯作者：
Miller,JH
中科院分区：
文献类型：
--
作者：
Hsia,HC;Lebkowski,JS;Leong,PM;Calos,MP;Miller,JH
We report the sequence changes in theEscherichia colilacIgene in 133 mutants detected after passage of an ultraviolet-irradiated shuttle vector human 293 cells. The results are compared with our previous studies of thelacIgene after ultraviolet light treatment inE. coli. In human cells, base substitutions predominate, and frameshifts are found much less frequently than in bacteria. The most frequent base change is the G·C to A·T transition. Overall. 110 of 112 transitions were G·C to A·T. Some of the hotspots seen inlacIin bacteria are prominent also in human 293 cells, suggesting that the same lesions are targeting mutations in both systems. Transitions are found almost exclusively at sequences at which pyrimidine-pyrimidine photoproducts can form. The data are consistent with the notion that a significant fraction of ultraviolet irradiation-induced mutagenesis in mammalian systems occurs by adding an A across from a photolesion. Double mutations are significantly more frequent in human cells than in bacteria. Reasons for this difference are discussed.
