Virus particle detection by solid phase immunocapture and atomic force microscopy
Virus particle detection by solid phase immunocapture and atomic force microscopy
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DOI：
10.1016/j.bbrc.2003.10.022
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发表时间：
2003-11-14
影响因子：
3.1
通讯作者：
Henderson, E
中科院分区：
文献类型：
--
作者：
Nettikadan, SR;Johnson, JC;Henderson, E
介绍了原子力显微镜（AFM）在快速、无标记检测和鉴定病毒中的一种新应用。使用“喷墨”蛋白质阵列技术构建多路复用的小型化抗体结构域。被称为“ViriChip”的固相亲和底物被用于噬菌体fd、犬细小病毒和柯萨奇病毒的免疫捕获，并通过AFM进行分析。发现免疫捕获具有抗体特异性，30 min内的灵敏度为10（8）pfu/ml。发现病毒结合在10（8）和10（10）pfu/ml之间呈线性，并且在4 h内未达到饱和。(C)2003年爱思唯尔公司All rights reserved.
A novel application of atomic force microscopy (AFM) in the rapid, label-free detection and identification of viruses is described. Multiplexed, miniaturized antibody domains were constructed using "ink-jet" protein arraying technology. The solid-phase affinity substrate termed the "ViriChip" was used in the immunocapture of bacteriophage fd, canine parvoviruses, and coxsackieviruses and analyzed by AFM. Immunocapture was found to be antibody-specific with a sensitivity of 10(8) pfu/ml in 30 min. Virus binding was found to be linear for concentration between 10(8) and 10(10) pfu/ml and did not reach saturation through 4 h. (C) 2003 Elsevier Inc. All rights reserved.