In vitro binding of matrix metalloproteinase-2 (MMP-2), MMP-9, and bacterial collagenase on collagenous wound dressings
In vitro binding of matrix metalloproteinase-2 (MMP-2), MMP-9, and bacterial collagenase on collagenous wound dressings
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DOI：
10.1111/j.1524-475x.2007.00263.x
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发表时间：
2007-07-01
影响因子：
2.9
通讯作者：
Friess, Wolfgang
中科院分区：
文献类型：
--
作者：
Metzmacher, Iris;Ruth, Peter;Friess, Wolfgang
慢性伤口的特征是伤口愈合反应失败以及伤口愈合延迟或不闭合。这导致临床治疗和/或家庭护理付出巨大努力。急性伤口和慢性伤口之间的主要区别是蛋白酶抑制剂和调节细胞外基质蛋白降解和再生的蛋白酶活性的不平衡。胶原蛋白和胶原蛋白/氧化再生纤维素敷料充当金属蛋白酶-2、基质金属蛋白酶-9 和细菌胶原酶中基质的竞争性底物，并对这种不平衡产生积极影响。批准用于慢性伤口治疗的两种伤口敷料（牛胶原蛋白 I 型海绵和氧化再生纤维素胶原海绵）在对所有酶的吸附特性上没有显着差异。一般来说，结合随着孵育时间的延长而增强。设备的密度和可通过制造工艺控制的可接触表面是伤口敷料效率的关键因素。
Chronic wounds are characterized by failure in wound-healing response and a delay in healing or nonclosure of the wounds. This results in a high effort in clinical treatment and/or home care. A major difference between acute wounds and chronic wounds is the imbalance of proteinase inhibitors and proteinase activity that regulates the degradation and regeneration of the extracellular matrix proteins. Collagen and collagen/oxidized regenerated cellulose dressings act as a competitive substrate for matrix in metalloproteinase-2, matrix metalloproteinase-9, and bacterial collagenase and influence this imbalance positively. Both wound dressings, approved for chronic wound treatment, the bovine collagen type I sponge and the oxidized regenerated cellulose collagen sponge, did not differ significantly in their sorption profiles for all enzymes. In general, binding was enhanced with a longer incubation time. The density of the device and the accessible surface, which can be controlled by the manufacturing process, are the crucial factors for the efficiency of the wound dressing.