Identification of the gene encoding the 5S ribosomal RNA maturase in Bacillus subtilis:: Mature 5S rRNA is dispensable for ribosome function
Identification of the gene encoding the 5S ribosomal RNA maturase in Bacillus subtilis:: Mature 5S rRNA is dispensable for ribosome function
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DOI：
10.1017/s1355838201002163
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发表时间：
2001-02-01
期刊：
影响因子：
4.5
通讯作者：
Putzer, H
中科院分区：
文献类型：
--
作者：
Condon, C;Brechemier-Baey, D;Putzer, H
25年前，Pace和他的同事描述了枯草杆菌细胞提取物中一种名为RNaseM5的活动，该活动负责5S核糖体RNA的成熟(Sogin&Pace，自然，1974,252：598-600)。在这里，我们证明了RNaseM5是由一个功能未知的基因编码的，该基因在低G+C革兰氏阳性细菌中高度保守。我们建议将该基因命名为rnmV。RnmV基因不是必需的。缺乏RNase M5的枯草杆菌菌株不能产生成熟的5S rRNA，这表明这一过程不是核糖体功能所必需的。然而，在游离和翻译核糖体中都可以发现5S rRNA前体。与RNase E不同的是，RNase E在单链区域裂解大肠杆菌5S前体，然后修剪产生成熟的5S RNA，而RNase M5在双链区域裂解枯草杆菌等效物，一步产生成熟的5S rRNA。在大多数情况下，真细菌含有一种或另一种产生5S rRNA的系统，沿着革兰氏阴性和革兰氏阳性线的分裂并不完美。探讨了RNase E或RNase M5的存在与预测的裂解位点的单链或双链性质之间的潜在关联。
Over 25 years ago, Pace and coworkers described an activity called RNase M5 in Bacillus subtilis cell extracts responsible for 5S ribosomal RNA maturation (Sogin & Pace, Nature, 1974, 252:598-600). Here we show that RNase M5 is encoded by a gene of previously unknown function that is highly conserved among the low G + C Gram-positive bacteria. We propose that the gene be named rnmV. The rnmV gene is nonessential. a. subtilis strains lacking RNase M5 do not make mature 5S rRNA, indicating that this process is not necessary for ribosome function. 5S rRNA precursors can, however, be found in both free and translating ribosomes. In contrast to RNase E, which cleaves the Escherichia coli 5S precursor in a single-stranded region, which is then trimmed to yield mature 5S RNA, RNase M5 cleaves the B. subtilis equivalent in a double-stranded region to yield mature 5S rRNA in one step. For the most part, eubacteria contain one or the other system for 5S rRNA production, with an imperfect division along Gram-negative and Gram-positive lines. A potential correlation between the presence of RNase E or RNase M5 and the single- or double-stranded nature of the predicted cleavage sites is explored.