Meiotic telomere clustering is inhibited by colchicine but does not require cytoplasmic microtubules
Meiotic telomere clustering is inhibited by colchicine but does not require cytoplasmic microtubules
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DOI：
10.1242/jcs.00055
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发表时间：
2002-10-01
影响因子：
4
通讯作者：
Cande, WZ
中科院分区：
文献类型：
--
作者：
Cowan, CR;Cande, WZ
端粒聚簇，花束的定义特征，是减数分裂前期的一个几乎普遍的特征，但其机制仍然未知。发现微管解聚剂秋水仙碱抑制花束形成。在秋水仙素处理的细胞端粒仍然分散在核周围，而未处理的细胞表现出突出的端粒簇。在花束形成后给予秋水仙素并不影响端粒的分散。秋水仙碱对花束形成的影响似乎是可分离的，其对细胞质微管的影响;甲基氨丙磷，一种高效的植物微管解聚药物，不影响端粒聚类。花束形成的抑制仅限于秋水仙碱和相关药物鬼臼毒素的各种微管解聚药物测试，这表明，在花束形成的目标参与具有结构特异性。
Telomere clustering, the defining feature of the bouquet, is an almost universal feature of meiotic prophase, yet its mechanism remains unknown. The microtubule-depolymerizing agent colchicine was found, to inhibit bouquet formation. Telomeres in colchicine-treated cells remained scattered in the nuclear periphery, whereas untreated cells exhibited a prominent telomere cluster. Colchicine administered after the bouquet had formed did not affect telomere dispersal. The effect of colchicine on bouquet formation appeared to be separable from its effect on cytoplasmic microtubules; amiprophos methyl, a highly effective plant microtubule-depolymerizing drug, did not affect telomere clustering. Inhibition of bouquet formation was limited to colchicine and the related drug podophyllotoxin out of the variety of microtubule-depolymerizing drugs tested, suggesting that the target involved in bouquet formation has a structural specificity.